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  4. Rat Platelet-derived growth factor DD(PDGF-DD) ELISA kit

Rat Platelet-derived growth factor DD(PDGF-DD) ELISA kit

  • 中文名稱：
    大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL017711RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒（CSB-EL017711RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-DD含量。PDGF - DD是一種血小板衍生生長因子異構(gòu)體。它在細(xì)胞的生長、增殖和遷移等過程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與受體結(jié)合后激活下游信號通路，影響細(xì)胞行為，在腫瘤、纖維化等疾病的發(fā)生發(fā)展中可能有潛在影響，是藥物研發(fā)的潛在靶點(diǎn)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域探究PDGF-DD在大鼠心血管疾病模型、器官纖維化機(jī)制、創(chuàng)傷修復(fù)實(shí)驗(yàn)中的動態(tài)表達(dá)特征，通過血清或血漿樣本可系統(tǒng)性評估循環(huán)系統(tǒng)中PDGF-DD水平，而組織勻漿檢測則有助于解析特定器官或病變部位的局部蛋白表達(dá)模式，為相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    PDGF-DD
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-90
    1:2Average %89
    Range %85-96
    1:4Average %90
    Range %84-96
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-103
    EDTA plasma (n=4)9590-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.744 2.678 2.711 2.531
    52.139 2.112 2.126 1.946
    2.51.509 1.476 1.493 1.313
    1.251.025 1.003 1.014 0.834
    0.6250.662 0.651 0.657 0.477
    0.3120.403 0.386 0.395 0.215
    0.1560.292 0.272 0.282 0.102
    00.181 0.179 0.180 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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