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  4. Rat Pro-opiomelanocortin(POMC) ELISA KIT

Rat Pro-opiomelanocortin(POMC) ELISA KIT

  • 中文名稱(chēng)：
    大鼠阿黑皮素原(POMC)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13569r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠阿黑皮素原(POMC)酶聯(lián)免疫試劑盒（CSB-E13569r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的POMC含量。POMC（阿黑皮素原）是一種重要的神經(jīng)肽前體蛋白。它在人體能量平衡、代謝調(diào)節(jié)、神經(jīng)內(nèi)分泌等方面發(fā)揮關(guān)鍵作用。研究機(jī)制主要聚焦于其裂解產(chǎn)生的多種活性肽，如促黑素、促腎上腺皮質(zhì)激素等，參與調(diào)節(jié)食欲、應(yīng)激反應(yīng)等生理過(guò)程。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，適用于神經(jīng)內(nèi)分泌研究、肥胖機(jī)制探索、下丘腦 - 垂體軸功能分析等科研領(lǐng)域，特別適合評(píng)估實(shí)驗(yàn)動(dòng)物模型在藥物干預(yù)、代謝調(diào)控或應(yīng)激狀態(tài)下POMC的表達(dá)水平變化。為研究能量平衡、攝食行為及相關(guān)疾病的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Pomc ELISA kit; Pomc2Pro-opiomelanocortin ELISA kit; POMC ELISA kit; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma ELISA kit; Gamma-MSH); Potential peptide; Corticotropin ELISA kit; Adrenocorticotropic hormone ELISA kit; ACTH); Melanocyte-stimulating hormone alpha ELISA kit; Alpha-MSH ELISA kit; Melanotropin alpha); Corticotropin-like intermediary peptide ELISA kit; CLIP); Lipotropin beta ELISA kit; Beta-LPH); Lipotropin gamma ELISA kit; Gamma-LPH); Melanocyte-stimulating hormone beta ELISA kit; Beta-MSH ELISA kit; Melanotropin beta); Beta-endorphin; Met-enkephalin] ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %99
    Range %93-105
    1:10Average %93
    Range %90-97
    1:20Average %95
    Range %90-100
    1:40Average %84
    Range %80-88
  • 回收率：
    The recovery of rat POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)8785-90
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.502 2.603 2.553 2.438
    2501.661 1.762 1.712 1.597
    1251.127 1.148 1.138 1.023
    62.50.724 0.713 0.719 0.604
    31.20.388 0.377 0.383 0.268
    15.60.287 0.276 0.282 0.167
    7.80.184 0.181 0.183 0.068
    00.115 0.114 0.115 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Stimulates the adrenal glands to release cortisol.; Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.; Increases the pigmentation of skin by increasing melanin production in melanocytes.; Endogenous orexigenic opiate.; Endogenous opiate.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    POMC family
  • 組織特異性：
    ACTH and MSH are produced by the pituitary gland.
  • 數(shù)據(jù)庫(kù)鏈接：