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  4. Rat Protein CYR61(CYR61) ELISA kit

Rat Protein CYR61(CYR61) ELISA kit

  • 中文名稱：
    大鼠CYR61蛋白(CYR61)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL006463RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠CYR61蛋白(CYR61)酶聯(lián)免疫試劑盒（CSB-EL006463RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CYR61含量。CYR61是一種細(xì)胞外基質(zhì)相關(guān)蛋白，在細(xì)胞增殖、分化、黏附等多種生理過程中發(fā)揮重要作用。研究發(fā)現(xiàn)，它與腫瘤的發(fā)生、發(fā)展、轉(zhuǎn)移密切相關(guān)，其機(jī)制可能涉及調(diào)節(jié)細(xì)胞信號(hào)通路，影響腫瘤微環(huán)境等，是潛在的腫瘤治療靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于心血管疾病模型、腫瘤機(jī)制研究、炎癥反應(yīng)評(píng)估或再生醫(yī)學(xué)等領(lǐng)域的基礎(chǔ)科研，通過檢測(cè)體液或組織樣本中CYR61的動(dòng)態(tài)變化，為探索其分子功能及信號(hào)通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccn1 ELISA kit; Cyr61 ELISA kit; Igfbp10CCN family member 1 ELISA kit; Cellular communication network factor 1 ELISA kit; Cysteine-rich angiogenic inducer 61 ELISA kit; Insulin-like growth factor-binding protein 10 ELISA kit; IBP-10 ELISA kit; IGF-binding protein 10 ELISA kit; IGFBP-10 ELISA kit; Protein CYR61 ELISA kit
  • 縮寫：
    CYR61
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-97
    1:2Average %95
    Range %91-99
    1:4Average %98
    Range %96-101
    1:8Average %99
    Range %96-104
  • 回收率：
    The recovery of rat CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)9690-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.625 2.529 2.577 2.413
    10002.062 1.956 2.009 1.845
    5001.384 1.366 1.375 1.211
    2500.871 0.881 0.876 0.712
    1250.551 0.539 0.545 0.381
    62.50.342 0.351 0.347 0.183
    31.250.249 0.255 0.252 0.088
    00.164 0.163 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 基因功能參考文獻(xiàn)：
    1. Compared with wild rats, Cyr61 was overexpressed in the lung tissue, pulmonary arterial and PASMCs in PAH rats. Exogenous recombinant Cyr61 protein promoted the proliferation of PASMCs in a dose-dependent manner. While the expression of Cyr61 in PASMCs was inhibited by specific siRNA, cell proliferation was restrained and the expression of p-AKT declined PMID: 28824319
    2. the matricellular protein CCN1 gene transfer induces hepatic stellate cell apoptosis through endoplasmic reticulum stress and unfolded protein response PMID: 26515130
    3. Cyclic AMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1. PMID: 25446180
    4. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
    5. In line with dermal fibrosis and scar formation, CCN1/CYR61 is involved in liver injury repair and tissue remodeling. CCN1/CYR61 gene transfer into extracellular matrix-producing liver cells is therefore potentially beneficial in liver fibrotic therapy PMID: 24487063
    6. Muscle progenitor cells treated with aging rodent serum caused CCN1 mRNA to increase by seven fold. PMID: 24196529
    7. Report assays with site-direct mutation analysis of CYR61/CCN1 promoter demonstrated that MRTF-A transactivated CYR61/CCN1 promoter mainly depending on CArG box. PMID: 23624342
    8. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
    9. Cyr61, an angiogenic factor with previously unknown neuronal function, is a novel regulator of dendritic growth. PMID: 23362279
    10. CYR-61 is important in the pathogenesis of rheumatoid arthritis, and SIRT-1/FoxO3a signaling is crucial to induction of CYR-61 in synovial fibroblasts. PMID: 23239110
    11. Endogenous CCN1 regulates proliferation of VSMCs and neointimal hyperplasia. Inhibition of CCN1 may provide a promising strategy for the prevention of restenosis after vascular interventions [CCN1]. PMID: 18388330
    12. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
    13. Suggest that CCN1, from the microenvironment of injured vessels, enhances reendothelialization via a direct action on endothelial progenitor cell differentiation, revealing a possible new mechanism underlying the process of vascular repair. PMID: 20830586
    14. Cyr61 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells. PMID: 20458273
    15. CYR61 is synthesized in arterial smooth muscle walls during proliferative restenosis PMID: 11897702
    16. induction of cyr61 is important for neuronal cell death in the central nervous system hippocampal progenitor cells, and JNK activation, but not of p38, as well as the subsequent SRF phosphorylation are involved in cyr61 gene induction PMID: 12576482
    17. after severe glomerular damage in anti-Thy-1 glomerulonephritis, Cyr61 may be involved in glomerular remodeling as a factor secreted from podocytes to inhibit mesangial cell migration. PMID: 12707386
    18. Overexpression of the cyr61 gene in stably transfected rat SMC (smooth muscle cells) line A7r5 inhibited the proliferation and DNA and protein synthesis. Cyr61 can also act as a growth inhibitor in SMC of genetically hypertensive rats. PMID: 12861037
    19. In a rat balloon carotid arterial injury model, CYR61 was rapidly induced in VSMCs in the early stage of injury and remained elevated until 14 days. PMID: 17234971
    20. The dissimilar growth characteristics between high and low Cx43 expressors may be due to differential regulation of CCN3 by varying levels of Cx43 PMID: 18004727
    21. Data suggest that matrix protein CCN1 may play an important role in microenvironment-mediated biological properties of endothelial progenitor cells . PMID: 18755182
    22. THBS1, Cyr61 and CTGF were all over-expressed in esophageal squamous cell carcinoma. PMID: 19698122
    23. Cyr61 is an early marker of extracellular matrix remodeling in rat bladder cells PMID: 12217894

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    CCN family
  • 數(shù)據(jù)庫(kù)鏈接：