Rat Pulmonary surfactant-associated protein A,SP-A ELISA Kit
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中文名稱:大鼠肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)酶聯(lián)免疫試劑盒
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貨號:CSB-E08684r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)酶聯(lián)免疫試劑盒(CSB-E08684r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SFTPA1含量。SFTPA1是一種肺表面活性蛋白,屬于C型凝集素亞家族,其基因突變與特發(fā)性肺纖維化有關(guān)。SFTPA1在表面活性蛋白穩(wěn)態(tài)和抵抗呼吸道病原體方面發(fā)揮重要作用,其機制研究涉及基因突變、可溶性介質(zhì)釋放、細(xì)胞凋亡失調(diào)等過程。試劑盒檢測范圍為31.25 pg/ml- 2000 pg/ml,靈敏度為7.8 pg/ml。適用于研究呼吸系統(tǒng)疾病模型中SP-A的動態(tài)變化,如肺纖維化、慢性阻塞性肺疾?。–OPD)的發(fā)病機制探索,或評估藥物干預(yù)對肺表面活性物質(zhì)代謝的影響本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
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縮寫:SFTPA1
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.25 pg/ml - 2000 pg/ml
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靈敏度:7.8 pg/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
88-99
1:2
Average %
93
Range %
86-98
1:4
Average %
99
Range %
95-104
1:8
Average %
97
Range %
93-100
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回收率:
The recovery of rat SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-98
EDTA plasma (n=4)
96
90-101
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2000
2.216
2.334
2.275
2.114
1000
1.808
1.767
1.788
1.626
500
1.365
1.328
1.347
1.185
250
0.971
0.939
0.955
0.794
125
0.652
0.621
0.637
0.475
62.5
0.429
0.453
0.441
0.280
31.25
0.287
0.284
0.286
0.124
0
0.165
0.158
0.162
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:SFTPA1是一種肺泡表面活性物質(zhì),最近的研究表明它可能在肺部疾病的治療中發(fā)揮著重要作用。一項研究表明,SFTPA1與慢性阻塞性肺疾?。–OPD)的嚴(yán)重程度有關(guān),因此可以用作COPD患者的預(yù)后指標(biāo)。另外,一些研究表明,SFTPA1可以作為潛在的肺癌診斷和治療的靶點。
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功能:In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
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基因功能參考文獻:
- SP-A expression was up-regulated in the middle ear effusion of non-typeable Haemophilus influenza otitis media. PMID: 30055742
- SPA binds dipalmitoyl-phosphatidylcholine, the major surfactant lipid component, but not phosphatidylinositol; SPD exhibits the opposite preference. Data suggest flexibility in a key surface loop supports distinctive lipid binding of SPA; quadruple mutant SPA (E171D/P175E/R197N/K203D) that introduces SPD-like loop-stabilizing Ca2+ binding site in carbohydrate recognition domain exhibits ligand-binding preferences of SPD. PMID: 28719181
- Downregulation of SP-A expression caused due to silica is an important factor in the occurrence and development of silicosis. PMID: 27660222
- Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
- Mutations of rat surfactant protein A have distinct effects on its glycosylation, secretion, aggregation and degradation. PMID: 25242514
- Dual positive effects of leptin were found on protein expression and transcriptional activity of thyroid transcription factor-1 PMID: 23894445
- VIP can up-regulate the expression of SP-A in alveolar type II cells. PMID: 21166190
- NG-nitro-L-arginine can protect the lung from LPS-induced injury by up-regulating the expression of PS. PMID: 20663300
- A novel role is demonstrated for SP-A in modulating endolysosomal trafficking via Rab7b (but not Rab5 nor Rab11) in primary alveolar macrophages, and the biochemical pathways are defined. PMID: 21248257
- SP-A stimulates the release of neutrophil chemotactic factor by alveolar type II cells, and this effect is mediated by the receptor for SP-A specifically expressed by these cells. PMID: 20473679
- VIP elevated SP-A expression in ATII cells which was mediated by enhanced sp-a gene transcription PMID: 20688121
- crystal structures of calcium-dependent complexes of the C-terminal neck and carbohydrate recognition domain of SP-A with carbohydrates PMID: 21047777
- The SPA level decreases significantly in acute pulmonary embolism. PMID: 16207426
- Data suggest that the pathway for secretion of newly synthesized surfactant protein-A is by transfer from the site of synthesis to the storage/secretory organelle prior to lamellar body exocytosis. PMID: 20382745
- the ability of SP-A to inhibit immune cell activation by Re-LPS may be due to its ability to block the binding of Re-LPS to LBP and prevent the initiation of the LBP/CD14 pathway for inflammatory reactions in the lung. PMID: 12204898
- SP-A selectively enhances mannose receptor expression on monocyte-derived macrophages, a process involving both the attached sugars and collagen-like domain of SP-A. PMID: 12244146
- SP-A can reverse the detrimental effects of surfactant oxidation on the biophysical properties of surfactant, by a mechanism that is dependent on interchain disulfide bond formation and the C-terminal domains of the protein. PMID: 12600986
- SP-A and SP-D are antimicrobial proteins that directly inhibit the growth of Histoplasma capsulatum by increasing permeability of the organism PMID: 12857753
- blocks secretagogue-stimulated phospholipid release, even in the presence of surfactant-like lipid PMID: 12882765
- Results suggest that there is no primary deficiency of surfactant proteins A, B, or C in the nitrofen-induced rat model of congenital diaphragmatic hernia. PMID: 12904592
- Crystal structure of trimeric carbohydrate recognition and neck domains of surfactant protein A PMID: 12913002
- SP-A enhances the phagocytosis of S. pneumoniae by alveolar macrophages through a CK2-dependent increase of cell surface SR-A localization (scavenger receptor a) PMID: 14993215
- SP-A and SP-D enhance mannose receptor-mediated phagocytosis of M. avium by macrophages PMID: 15187139
- Differential allele-specific expression was observed in all tissues studied (OPN) or in all extrapulmonary tissues (SP-A). Double heterozygous rats revealed lack of coordinate allele expression among SP-A, SP-D, and OPN. PMID: 15482851
- NO mediated the decrease in surfactant protein gene expression PMID: 15640287
- We suggest that enhanced release of surfactant phospholipids and SP-A represents an early protective response that compensates in part for the inactivation of intraalveolar surfactant in the early phase of IR injury. PMID: 15969762
- The addition of rat or recombinant rat SP-A to human monocyte-derived macrophages significantly raises the level of cytosolic calcium above baseline within 10 seconds of SP-A addition PMID: 16081790
- SP-A and Prdx6 directly interact, which provides a mechanism for regulation of the PLA(2) activity of Prdx6 by SP-A PMID: 16330552
- results indicated SP-A & SP-D have distinct functions in lung homeostasis & the function of the neck domain & carbohydrate recognition domain of SP-D is dependent on its own NH2-terminal & collagenous domains that cannot be complemented by those of SP-A PMID: 16500946
- Direct effect of SFTPA1 on rat myometrial cells and inhibitory cross talk between SFTPA1 and LPS signals. PMID: 17202387
- SP-A2 enhances phagocytosis of Pseudomonas aeruginosa by rat alveolar macrophages more than does SP-A1. PMID: 17220308
- Air spaces of neonatal lungs comprised lower fractions of tubular myelinand increased unilamellar vesicles, which correlated with low SP-A concentrations in lung lavage fluid and increased respiratory rates, respectively PMID: 17469149
- alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412
- cigarette smoke alone decreases the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways PMID: 18480979
- After OVA challenge alveolar epithelial cells Type II (AEII) show a significantly higher expression of SP-A and SP-D leading also to higher amounts of both SPs in BALF, and macrophages gather predominantly SP-A. PMID: 18802356
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
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蛋白家族:SFTPA family
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