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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat Resistin ELISA Kit
  • 中文名稱(chēng)：
    大鼠抵抗素(Resistin)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06885r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠抵抗素(Resistin)酶聯(lián)免疫試劑盒（CSB-E06885r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Resistin含量。Resistin 是一種脂肪細(xì)胞因子，它與肥胖、胰島素抵抗和炎癥等密切相關(guān)。研究發(fā)現(xiàn)，其水平在肥胖個(gè)體中往往升高。機(jī)制上，它可能通過(guò)干擾胰島素信號(hào)通路、激活炎癥信號(hào)等，在代謝紊亂及相關(guān)疾病進(jìn)程中發(fā)揮重要作用。試劑盒檢測(cè)范圍為125 pg/mL-8000 pg/mL，適用于科研領(lǐng)域中對(duì)大鼠代謝疾病模型（如2型糖尿病、肥胖癥）的機(jī)制研究、藥物干預(yù)效果評(píng)估或Resistin表達(dá)調(diào)控實(shí)驗(yàn)，可通過(guò)檢測(cè)不同生理病理狀態(tài)下血清、組織等樣本的Resistin水平，揭示其在能量代謝與炎癥反應(yīng)中的作用。試劑盒提供完整組分及操作說(shuō)明書(shū)，確保實(shí)驗(yàn)穩(wěn)定性和重復(fù)性，滿(mǎn)足基礎(chǔ)研究與生物標(biāo)志物探索需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Resistin
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %96
    Range %91-101
    1:4Average %95
    Range %90-101
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of rat resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-94
    EDTA plasma (n=4)9995-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.452 2.351 2.402 2.321
    40001.601 1.601 1.601 1.520
    20001.024 1.024 1.024 0.943
    10001.501 0.534 1.018 0.937
    5000.993 0.314 0.654 0.573
    2500.497 0.205 0.351 0.270
    1250.127 0.121 0.124 0.043
    00.082 0.080 0.081 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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