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  4. Rat Rheumatoid Factor(RF) ELISA Kit

Rat Rheumatoid Factor(RF) ELISA Kit

  • 中文名稱：
    大鼠類風(fēng)濕因子(RF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13666r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠類風(fēng)濕因子(RF)酶聯(lián)免疫試劑盒（CSB-E13666r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的RF含量。大鼠類風(fēng)濕因子（RF）是一種自身免疫性疾病類風(fēng)濕關(guān)節(jié)炎（RA）的標(biāo)志物，其研究機(jī)制涉及免疫系統(tǒng)的異常激活，包括抗原識別、T細(xì)胞介導(dǎo)的炎癥反應(yīng)和軟骨破壞。通過建立動物模型，研究者可以模擬人類RA的病理特征，從而探索發(fā)病機(jī)制和開發(fā)新的治療策略。試劑盒檢測范圍為15.6 mIU/mL-1000 mIU/mL，該產(chǎn)品適用于科研場景下大鼠血清或血漿樣本的RF水平檢測，如關(guān)節(jié)炎動物模型的炎癥程度分析、免疫調(diào)節(jié)劑篩選實驗或基礎(chǔ)醫(yī)學(xué)研究中自身免疫反應(yīng)動態(tài)監(jiān)測，為研究者提供穩(wěn)定可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    RF
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 mIU/mL-1000 mIU/mL
  • 靈敏度：
    3.9 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %104
    Range %96-108
    1:10Average %94
    Range %91-98
    1:20Average %102
    Range %98-106
    1:40Average %88
    Range %82-96
  • 回收率：
    The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8983-95
    EDTA plasma (n=4)9992-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    10002.646 2.545 2.596 2.443
    5001.975 2.076 2.026 1.873
    2501.284 1.308 1.296 1.143
    1250.751 0.762 0.757 0.604
    62.50.512 0.501 0.507 0.354
    31.20.353 0.342 0.348 0.195
    15.60.285 0.281 0.283 0.130
    00.153 0.152 0.153 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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