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  4. Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Rat Runt-related transcription factor 2(RUNX2) ELISA kit

  • 中文名稱：
    大鼠Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020594RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)酶聯(lián)免疫試劑盒（CSB-EL020594RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RUNX2含量。RUNX2是重要的轉(zhuǎn)錄因子，在骨骼發(fā)育和骨代謝中起關(guān)鍵作用。其能調(diào)控成骨細胞分化和軟骨細胞成熟。研究機制主要聚焦于它與多種信號通路相互作用，如BMP、Wnt等，進而對骨形成相關(guān)基因表達進行調(diào)節(jié)。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于體外科研實驗中探索骨骼發(fā)育機制、骨代謝調(diào)控、骨質(zhì)疏松模型評估、骨再生修復(fù)研究以及干細胞向成骨細胞分化的動態(tài)監(jiān)測等領(lǐng)域。為骨生物學(xué)、骨組織工程及骨疾病機制研究提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 91-103  
    1:2 Average % 102  
    Range % 94-107  
    1:4 Average % 88  
    Range % 83-91  
    1:8 Average % 94  
    Range % 84-98  
  • 回收率：
    The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 103 93-107  
    EDTA plasma (n=4) 98 90-102  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    200 2.551 2.452 2.502 2.322  
    100 2.126 1.996 2.061 1.881  
    50 1.359 1.341 1.350 1.170  
    25 0.793 0.818 0.806 0.626  
    12.5 0.474 0.481 0.478 0.298  
    6.25 0.395 0.403 0.399 0.219  
    3.12 0.266 0.257 0.262 0.082  
    0 0.182 0.178 0.180    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
  • 亞細胞定位：
    Nucleus.
  • 數(shù)據(jù)庫鏈接：