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  4. Rat Sclerostin(SOST) ELISA kit

Rat Sclerostin(SOST) ELISA kit

  • 中文名稱：
    大鼠硬骨素(SOST)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL022415RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠硬骨素(SOST)酶聯(lián)免疫試劑盒（CSB-EL022415RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SOST含量。SOST是一個重要靶點。它編碼的骨硬化蛋白可抑制成骨細(xì)胞活性，影響骨形成。目前對其的研究聚焦于揭示調(diào)控骨代謝的機(jī)制，旨在通過干預(yù)SOST，為骨質(zhì)疏松等骨疾病開發(fā)新療法，改善骨密度和骨質(zhì)量。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于骨骼發(fā)育、骨修復(fù)模型構(gòu)建、代謝性疾病動物實驗等科研場景，能夠?qū)λ幬锔深A(yù)、基因編輯或病理狀態(tài)下SOST的表達(dá)水平變化進(jìn)行動態(tài)分析，為骨生物學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sost ELISA Kit; Sclerostin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %93-101
    1:2Average %92
    Range %86-99
    1:4Average %90
    Range %88-94
    1:8Average %101
    Range %96-103
  • 回收率：
    The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9794-103
    EDTA plasma (n=4)105105-109
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.362 2.545 2.454 2.316
    6001.588 1.686 1.637 1.499
    3001.123 1.102 1.113 0.975
    1500.767 0.744 0.756 0.618
    750.520 0.546 0.533 0.395
    37.50.304 0.324 0.314 0.176
    18.750.248 0.241 0.245 0.107
    00.136 0.140 0.138
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 功能：
    Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
  • 基因功能參考文獻(xiàn)：
    1. alleviated the osteogenic differentiation of ectomesenchymal stem cells (EMSCs), and LNGFR enhanced the osteogenic differentiation of EMSCs by decreasing SOST PMID: 29226516
    2. TNF-alpha might mediate alveolar bone loss via inducing expression of osteocytic RANKL and sclerostin in type 1 diabetes rats with periodontitis PMID: 29240821
    3. These data suggest that sclerostin plays an important role in the bone remodeling of tooth movement. PMID: 28081119
    4. a direct correlation between attenuated SOST expression and an increase in the osteogenic potential of UMR-106 cells, is reported. PMID: 27233518
    5. In conclusion, naringin could prevent progress of disuse osteoporosis in rats, which may be mediated by increased periostin expression and subsequently inhibition of sclerostin and activation of Wnt/beta-catenin signaling pathways. PMID: 26541456
    6. Scl-Ab increased osteoblast surface and bone formation, indicating direct bone anabolic effects, whereas TE reduced osteoclast surface with minimal effect on bone formation, indicating antiresorptive effects PMID: 25359699
    7. Data indicate that sclerostin (SOST) gene expression is negatively regulated by histone deacetylase HDAC5 and positively by class I HDACs. PMID: 25012661
    8. The results suggest that Sost mRNA expression in metaphyseal bone responds to mechanical unloading in an opposite direction to that observed in diaphyseal cortical bone. PMID: 23337040
    9. Pharmacologic inhibition of sclerostin with Scl-Ab has no impact on articular cartilage remodeling in rats with posttraumatic osteoarthritis. PMID: 23233270
    10. Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from 3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. PMID: 22766096
    11. The proportion of sclerostin-positive osteocytes in cortical bone was significantly higher. PMID: 22174402
    12. Insulin-deficiency in insulin-dependent diabetes mellitus decreases osteoblastogenesis associated with inhibition of Wnt signaling through the increased expression of Sost and Dkk1 and the inhibition of Akt activation. PMID: 21567076
    13. These results indicate that sclerostin inhibition by treatment with a sclerostin antibody increased bone formation, bone mass, and bone strength in aged male rats. PMID: 20641040
    14. SOST regulation may play a role in mediating PTH action in bone PMID: 15946907
    15. The anabolic effects of parathyroid hormone (PTH) are in line with the fall of SOST mRNA and protein in all the three bone segments examined; the rise of bone turnover supports a negative role of SOST in bone formation. PMID: 17549589
    16. SOST expression in osteocytes of adult bone and its inhibition by PTH is mediated by MEF2A, C, and D transcription factors controlling the SOST bone enhancer. PMID: 17696759
    17. Modulation of sclerostin levels appears to be a finely tuned mechanism by which osteocytes coordinate regional and local osteogenesis in response to increased mechanical stimulation, perhaps via releasing the local inhibition of Wnt/Lrp5 signaling PMID: 18089564

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Sclerostin family
  • 數(shù)據(jù)庫鏈接：