Rat Serum amyloid A,SAA ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒（CSB-E08590r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SAA含量。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于炎癥機(jī)制研究、感染性疾病模型評(píng)估、免疫調(diào)節(jié)藥物開發(fā)等科研領(lǐng)域，例如在實(shí)驗(yàn)性關(guān)節(jié)炎、膿毒癥或腫瘤模型中動(dòng)態(tài)監(jiān)測SAA水平變化，為病理機(jī)制解析及治療干預(yù)效果評(píng)價(jià)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

SAA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 ng/mL-5000 ng/mL
靈敏度：

19.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	94
	Range %	91-97
1:4	Average %	100
	Range %	97-103
1:8	Average %	102
	Range %	99-105

回收率：

The recovery of rat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	98	95-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.