Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit
-
中文名稱:大鼠可溶性凝集素樣氧化低密度脂蛋白受體1(sLOX-1)酶聯(lián)免疫試劑盒
-
貨號(hào):CSB-E15845r
-
規(guī)格:96T/48T
-
價(jià)格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:大鼠可溶性凝集素樣氧化低密度脂蛋白受體1(sLOX-1)酶聯(lián)免疫試劑盒(CSB-E15845r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的OLR1含量。OLR1是一種重要靶點(diǎn)。它在動(dòng)脈粥樣硬化、炎癥等病理過程中扮演關(guān)鍵角色。研究機(jī)制方面,主要聚焦于其與氧化低密度脂蛋白的結(jié)合,引發(fā)細(xì)胞內(nèi)一系列信號(hào)傳導(dǎo),影響細(xì)胞功能,目前圍繞其開發(fā)干預(yù)措施以治療相關(guān)疾病的研究不斷推進(jìn)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于心血管疾病模型研究、代謝綜合征機(jī)制探索及藥物干預(yù)效果評(píng)估等科研場景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名:Olr1; Lox1; Oldlr1; Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1
-
縮寫:
-
Uniprot No.:
-
種屬:Rattus norvegicus (Rat)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測范圍:0.156 ng/mL-10 ng/mL
-
靈敏度:0.039 ng/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領(lǐng)域:Cardiovascular
-
測定原理:quantitative
-
測定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat sLOX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-94 1:2 Average % 92 Range % 88-99 1:4 Average % 88 Range % 84-92 1:8 Average % 95 Range % 90-100 -
回收率:
The recovery of rat sLOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 85 80-90 EDTA plasma (n=4) 94 90-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.375 2.227 2.301 2.190 5 1.816 1.902 1.859 1.748 2.5 1.240 1.208 1.224 1.113 1.25 0.725 0.785 0.755 0.644 0.625 0.421 0.439 0.430 0.319 0.312 0.263 0.284 0.274 0.163 0.156 0.218 0.221 0.220 0.109 0 0.109 0.112 0.111 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能:Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
-
基因功能參考文獻(xiàn):
- an important role of LOX-1 in the maintenance of PASMCs phenotype. PMID: 29069578
- data support an important role for STBEVs in impairment of vascular function via activation of LOX-1 and reduced nitric oxide mediated vasodilation. Moreover, we postulate that the LOX-1 pathway could be a potential therapeutic target in pathologies associated with vascular dysfunction during pregnancy PMID: 28672042
- Negative feedback regulation between let-7g and LOX-1 mediated hypoxia-induced proliferation of in pulmonary artery smooth muscle cells (PASMCs). PMID: 28108289
- LOX-1 promotes right ventricular hypertrophy in hypoxia-exposed rats PMID: 28259654
- Losartan attenuated lung injury by suppressing LOX-1 expression to alleviate inflammation and cell apoptosis. PMID: 26884836
- Swimming exercise reduced heart expression of LOX-1 receptor and free radical production in experimental diabetes. PMID: 26432573
- OLR1 is a novel molecular link between the proliferative and inflammatory responses of vascular smooth muscle cells. PMID: 26305474
- data indicated that olmesartan may attenuate the impairment of endothelial cell via down-regulation of the increased LOX-1 expression induced by ox-LDL. PMID: 22408405
- EETs exert beneficial effects on ox-LDL-induced inflammation primarily through the inhibition of LOX-1 receptor upregulation, MAPK phosphorylation, and NF-kappaB activation and through the upregulation of CYP2J4 expression. PMID: 24486707
- LOX-1 is a promising therapeutic target in neonatal HIE, and the inhibition of LOX-1 may become a novel treatment for babies who have experienced asphyxia. PMID: 24731447
- results indicate that LOX-1 is essential in microglia for promoting an inflammatory response in the presence of soluble neuronal-injury signals such as extracellular HSP60, thereby linking neuroinflammation and neurotoxicity PMID: 22847064
- LOX-1 activation by oxLDL and subsequent peroxynitrite generation as a novel mechanism by which disruption of the BBB occurs in EPE. PMID: 23230281
- These findings indicated that LOX-1 expression was up-regulated in the cortex of SHR and its expression has implication in neuronal apoptosis. PMID: 22885180
- In a rat model of preeclampsia, there is dramatic increase in expression levels of both LOX-1 receptor and endothelial NO synthase enzyme, along with increased superoxide production and modestly decreased endothelial function. PMID: 22392901
- Inhibition of rat LOX-1 receptor reduces leukocyte adhesion in experimental endotoxemia. PMID: 21143965
- Data show that a high fat diet elevates LOX-1 protein and mRNA expression in aorta, and that sleeve gastrectomy decreases plasma LDL levels, and downregulates LOX-1 protein and mRNA expression. PMID: 21990956
- LOX-1 appears to be involved in CRP-induced complement activation, and thus may serve to locate the site of CRP-induced complement activation and inflammation. PMID: 21821723
- enhanced expression and activation of LOX-1 mediated the enhancement of vascular permeability, which contributed to the vascular lipid accumulation under hypertensive states PMID: 20216085
- expression pattern of LOX1-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
- findings indicate that myocardial ischemia-reperfusion upregulates LOX-1 expression, which through p38 MAPK activation increases the expression of MMP-1 and adhesion molecules PMID: 12384456
- LOX-1 may have a role in the pathogenesis of osteoarthritis, inducing chondrocyte death through PI3 kinase/Akt pathway. PMID: 12435393
- LOX-1 is an adhesion molecule involved in leukocyte recruitment and may represent an attractive target for modulation of endotoxin-induced inflammation PMID: 12538855
- enhanced renal expression of olr1 might play some roles in the progression of chronic renal failure in rats. PMID: 12661921
- LOX-1 up-regulation is redox-sensitive PMID: 16055083
- the bradykinin-eNOS and oxidative stress-LOX-1 pathway have roles in cardiovascular remodeling under chronic angiotensin-converting enzyme inhibition PMID: 16214149
- LOX-1 activation driven by lipid oxidants in the preurine fluid is critical in inflammatory changes PMID: 18322020
- Oxidized low-density lipoprotein induces matrix metalloproteinase-9 expression at transcriptional and translational level in brain astrocytes, which are mediated through mitogen-activated protein kinase signaling pathways leading to AP-1 activation. PMID: 18661553
顯示更多
收起更多
-
亞細(xì)胞定位:Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted.
-
組織特異性:Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.
-
數(shù)據(jù)庫鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-