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Rat Somatostatin,SS ELISA Kit

  • 中文名稱:
    大鼠生長抑素(SS)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08204r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠生長抑素(SS)酶聯(lián)免疫試劑盒(CSB-E08204r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SST含量。SST即生長抑素,它是一種廣泛存在于中樞神經(jīng)系統(tǒng)和胃腸道等部位的神經(jīng)肽。其背景在于能抑制多種激素分泌。研究機制方面,它與生長抑素受體結(jié)合,調(diào)控細胞增殖、內(nèi)分泌等功能,在治療內(nèi)分泌腫瘤、胃腸道疾病等領(lǐng)域有重要研究價值。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于研究SS在大鼠模型中的生理調(diào)控機制,如胃腸道功能研究、垂體激素關(guān)聯(lián)性分析或神經(jīng)內(nèi)分泌系統(tǒng)實驗,為代謝性疾病、應(yīng)激反應(yīng)及神經(jīng)退行性研究提供定量數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Sst ELISA Kit; Smst ELISA Kit; Somatostatin ELISA Kit; somatotropin release-inhibiting factor ELISA Kit; SRIF) [Cleaved into: Antrin; Somatostatin-28; Somatostatin-14; Neuronostatin ELISA Kit; NST)] ELISA Kit
  • 縮寫:
    SST
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.39 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %94
    Range %92-98
    1:2Average %97
    Range %92-99
    1:4Average %92
    Range %87-96
    1:8Average %89
    Range %82-94
  • 回收率:
    The recovery of rat SS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)9892-106
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.229 2.128 2.179 1.978
    501.638 1.557 1.598 1.397
    251.112 1.101 1.107 0.906
    12.50.784 0.773 0.779 0.578
    6.250.496 0.485 0.491 0.290
    3.120.394 0.405 0.400 0.199
    1.560.303 0.307 0.305 0.104
    00.200 0.201 0.201  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.; May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
  • 基因功能參考文獻:
    1. Results suggest that somatostatin suppresses GABA release from the amacrine cells by inhibiting presynaptic Ca2+ channels, in part through activating sst5/sst2 receptors, a process that is mediated by the intracellular cAMP-PKA signaling pathway PMID: 24846611
    2. These results suggest that a local release of somatostatin facilitates the excitability of trigeminal nocicepitve C1 neuronal activity via inhibition of GABAergic neurons PMID: 24321530
    3. when activated by SST-14, SSTR2A internalizes and recycles via the Golgi, which requires ECE-1 degradation of SST-14 and receptor dissociation from beta-arrestins PMID: 23913690
    4. Helicobacter pylori is capable of inhibiting acid secretion directly as well as indirectly by activating intramural CGRP sensory neurons coupled to stimulation of SST and inhibition of histamine secretion. PMID: 23392237
    5. Data suggest that neuronostatin action to reduce food intake and water intake is mediated through melanocortin 3/4 receptors, not through oxytocin receptors. PMID: 20600426
    6. both ferret odor exposure and elevated plus maze exposure resulted in increased somatostatin PMID: 22917777
    7. This study provides structural evidence that SST immunoreactivity in the pre-BotC is highly expressed in somas, terminals, and primary dendrites, and overlapped with small fusiform NK1R-ir neurons. PMID: 22765158
    8. Surviving somatostatin interneurons display increased mRNA levels at late intervals (3 months) after kainic acid-induced epilepsy. PMID: 22437342
    9. Neuronostatin, a novel peptide encoded by somatostatin gene, regulates cardiac contractile function and cardiomyocyte survival. PMID: 22170057
    10. These findings suggest that somatostatin directly inhibits the activity of rat GnRH neurons through volume transmission in the organum vasculosum of the lamina terminalis region. PMID: 22147011
    11. 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway is suppressed in the water-immersion restraint stressed rats PMID: 21291881
    12. Pancreatic islet CCK1/2 receptors initiate somatosatin secretion through constitutive secretion controlled at the level of protein synthesis. PMID: 19959964
    13. pancreatic release of somatostatin inhibited by ghrelin PMID: 11834435
    14. data suggest that methionine enkephalin, neurotensin and somatostatin are released in the caudal trigeminal nucleus after electrical stimulation of the trigeminal ganglion, an experimental model of migraine PMID: 12080490
    15. High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
    16. The remarkable increase in somatostatin immunoreactivity in the aged rat suprachiasmatic nucleus reveals that not all suprachiasmatic neuropeptidergic systems decline with age, and shows an age-related role for somatostatin in the suprachiasmatic nucleus. PMID: 12470886
    17. Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
    18. antral atrial natriuretic peptide (ANP) and somatostatin (SST) secretion linked by paracrine feedback pathways: endogenous ANP acting via the NPR-A receptor stimulates SST secretion; endogenous SST acting via the sst2 receptor inhibits ANP secretion PMID: 12527142
    19. adrenomedullin, acting via intramural fundic neurons, stimulates somatostatin and thus inhibits histamine and acid secretion PMID: 12573799
    20. Presence and cellular localization of somatostatin in the three lipid-containing lacrimal glands of the rat. PMID: 12613892
    21. ghrelin secretion from the stomach is regulated by gastric somatostatin PMID: 12615065
    22. fasting for 48 hours increases somatostatin synthesis and receptor subtype expression PMID: 12832106
    23. Somatostatin is involved in the transcriptional regulation of the GH gene, and that the effect of SRIF is mediated through pertussis toxin-sensitive G protein PMID: 14664705
    24. L-glutamate triggers somatostatin secretion from delta-cells by way of the GluR4c-flip receptor under low-glucose conditions. The released somatostatin may complete the feedback inhibition of alpha-cells. PMID: 15220198
    25. A second pathway is required to induce changes in srif and sst2 expression, since constitutively active calcineurin alone is not sufficient to induce these changes. PMID: 16000155
    26. Somatostatin-immunoreactive terminals particularly targeted intrinsic cardiac neurons that were immunoreactive for calbindin PMID: 16374620
    27. These findings suggest that inhibitory inputs from SOM+ interneurons may interact with excitatory inputs to pyramidal cell distal dendrites in the BLa. These interactions might affect synaptic plasticity related to emotional learning. PMID: 17120289
    28. only bistratified cells express the neuropeptide somatostatin PMID: 17122364
    29. Sex-specific, transient transcription of the somatostatin gene may causally relate to the estrogen-dependent organization of the Sexually Dimorphic Nucleus-Preoptic area. PMID: 17138650
    30. The adrenergic system is not involved in producing HISS-dependent insulin resistance in response to hemorrhage. Somatostatin appears to be the hormonal regulator of this response; that the somatostatin derives from a neural origin within the liver PMID: 17202832
    31. RT-PCR and immunocytochemistry studies support the presence of somatostatin in rat Kupffer cells. PMID: 17481746
    32. our data anticipate the possibility that carboxypeptidase-E, prohormone convertase 1, and prohormone convertase 2 might be potential target for the maturation of somatostatin PMID: 17543468
    33. Somatostatin and glucagon pulses were coupled in antisynchronous fashion, supporting the idea that the delta-cells have a local inhibitory effect on glucagon release. PMID: 17628719
    34. Present results strongly support the neuroinflammation as a potential factor involved in the age-related degeneration of somatostatin GABAergic cells. PMID: 17666053
    35. SS-14 is not involved in the control of the physiologic involution of the thymus, though it induces thymic weight loss through the reduction of true lymphoid tissue; SS-14 modulates thymocytes development and maturation PMID: 17761280
    36. Endogenously released somatostatin may induce slow inhibitory postsynaptic currents through the activation of GIRK channels in substantia gelatinosa neurons; this slow synaptic transmission might play an important role in spinal antinociception. PMID: 18356203
    37. Taken together, these results provide first evidence that the presence of SST is a unique feature for the selective sparing of medium sized aspiny interneurons in excitotoxicity. PMID: 18483877
    38. Data show that SOM neurons in the claustrum do not reveal any significant changes in their numerical density during the postnatal period. PMID: 18598846
    39. Inhibitory effect of Sst on prostaglandin E2 synthesis by primary neonatal rat glial cells is reported. PMID: 18619496
    40. SST-14 was cointernalized with SSTR3, was uncoupled from receptor, & was sorted into an endocytic degradation pathway, whereas octreotide was recycled as an intact peptide. Chronic stimulation of SSTR3 induced time-dependent downregulation of receptor. PMID: 18702662
    41. Somatostatin secretion in perfused rat pancreas by obestatin PMID: 18773927
    42. Release of regulatory gut peptides somatostatin, by acid and hyperosmolal solutions in the intestine in conscious rats PMID: 18992283
    43. These data support involvement of SRIF2B (SSTR4) receptors in the negative contractile effects of SRIF-14, while one or more of the three SRIF1 receptor subtypes (SSTR2, 3 or 5) may contribute to the positive contractile effects of SRIF-14. PMID: 19088447
    44. Somatostatin has no effect on basal and IL-1beta-stimulated PGE(2) release, whereas cortistatin reduced baseline prostanoids production and abolished stimulation elicited by IL-1beta. PMID: 19552966
    45. These results provide evidence that somatostatin tonically inhibits the peripheral nerve terminals of small-diameter cutaneous afferent fibers. PMID: 19596279

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Somatostatin family
  • 組織特異性:
    Somatostatin is detected in neurons throughout the brain, including in the lateral septum, nucleus accumbens, amygdaloid complex, hypothalamic periventricular nucleus, hippocampus, cortex, cerebellum and several brainstem nuclei (at protein level). Neuron
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