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  4. Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit

Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit

  • 中文名稱：
    大鼠類固醇5α還原酶1 (SRD5A1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14053r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠類固醇5α還原酶1 (SRD5A1)酶聯(lián)免疫試劑盒（CSB-E14053r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SRD5A1含量。SRD5A1即5α-還原酶1型，在多種組織中表達。它能將睪酮轉(zhuǎn)化為活性更強的雙氫睪酮。對其研究機制主要圍繞調(diào)節(jié)雄激素活性展開，在前列腺疾病、脫發(fā)等病癥的發(fā)生發(fā)展中扮演角色，為相關(guān)治療藥物研發(fā)提供了重要靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于實驗室研究中對SRD5A1表達水平的動態(tài)監(jiān)測，如內(nèi)分泌代謝研究、藥物干預實驗或疾病模型構(gòu)建中酶活性變化的定量分析。為探索SRD5A1在生理及病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Srd5a1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
  • 縮寫：
    SRD5A1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %100
    Range %95-105
    1:10Average %96
    Range %90-102
    1:20Average %94
    Range %88-99
    1:40Average %90
    Range %85-95
  • 回收率：
    The recovery of rat SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10198-105
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.762 2.803 2.783 2.589
    5002.117 2.104 2.111 1.917
    2501.598 1.583 1.591 1.397
    1251.103 1.136 1.120 0.926
    62.50.652 0.672 0.662 0.468
    31.250.416 0.426 0.421 0.227
    15.60.341 0.351 0.346 0.152
    00.193 0.195 0.194 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • 基因功能參考文獻：
    1. Ziram inhibited SRD5A1 and AKR1C14 activities, thus possibly interfering with neurosteroid production in rats. PMID: 28707553
    2. Exposure of middle-aged rats to environmental enrichment increased mRNA levels of 5al;pha-reductase-1, 3alpha-HSD and cytochrome P450 17alpha-hydroxylase/c17,20-lyase and decreased the methylation state of the 5alpha-reductase-1 gene PMID: 26021641
    3. Low Srd5a1 expression is associated with benign prostatic hypertrophy. PMID: 23405234
    4. Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
    5. Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
    6. Elevated levels of 5alpha-reduced androgens seen in hyperinsulinemic conditions might be explained on the basis of a stimulatory effect of insulin on 5alpha-reductase in granulosa cells. PMID: 20810561
    7. Thus, the present results indicate that the vitamin E-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation. PMID: 20447326
    8. 5 alpha-R1 immunostaining was detected mainly in the white matter (Wm). PMID: 15305365
    9. great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
    10. Our results demonstrate that both 5alpha-R isozymes are significantly higher in prefrontal cortex of male rats after acute swim stress in comparison with control rats PMID: 17826869
    11. Our results suggest that 5AR inhibitors elicit antipsychotic-like effects in animals and may be proposed as a putative novel target in the management of psychotic disorders. PMID: 18354385

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  • 亞細胞定位：
    Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Steroid 5-alpha reductase family
  • 組織特異性：
    Liver and prostate (at a low level).
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:24950

    STRING: 10116.ENSRNOP00000023659

    UniGene: Rn.4620