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  4. Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

  • 中文名稱：
    大鼠雌激素硫酸轉(zhuǎn)移酶(SULT1E1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13472r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠雌激素硫酸轉(zhuǎn)移酶(SULT1E1)酶聯(lián)免疫試劑盒（CSB-E13472r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SULT1E1含量。SULT1E1 屬于磺基轉(zhuǎn)移酶家族。其背景在于參與體內(nèi)多種內(nèi)源性和外源性物質(zhì)的磺化代謝。研究機(jī)制方面，主要是催化底物與 3'-磷酸腺苷 5'-磷酸硫酸（PAPS）發(fā)生磺化反應(yīng)，改變底物活性、水溶性等，在激素調(diào)節(jié)、藥物代謝等過程中有重要作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研場景中探索SULT1E1在激素代謝通路中的作用，例如評估卵巢、肝臟等組織中的酶活性變化，或分析藥物干預(yù)、疾病模型下雌激素硫酸化水平波動。為生殖生物學(xué)、藥理學(xué)及內(nèi)分泌領(lǐng)域的基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Sult1e1 ELISA Kit; Ste ELISA Kit; Ste1 ELISA Kit; Sulfotransferase 1E1 ELISA Kit; ST1E1 ELISA Kit; EC 2.8.2.4 ELISA Kit; Estrogen sulfotransferase ELISA Kit; isoform 1 ELISA Kit; EST-1 ELISA Kit; Estrone sulfotransferase ELISA Kit; Sulfotransferase ELISA Kit; estrogen-preferring ELISA Kit
  • 縮寫：
    SULT1E1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SULT1E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-93
    1:2Average %108
    Range %99-111
    1:4Average %92
    Range %87-97
    1:8Average %99
    Range %92-103
  • 回收率：
    The recovery of rat SULT1E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9487-97
    EDTA plasma (n=4)9990-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.488 2.377 2.433 2.300
    52.075 2.045 2.060 1.927
    2.51.412 1.389 1.401 1.268
    1.250.916 0.898 0.907 0.774
    0.6250.574 0.564 0.569 0.436
    0.3120.353 0.367 0.360 0.227
    0.1560.267 0.260 0.264 0.131
    00.134 0.132 0.133
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.
  • 亞細(xì)胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Sulfotransferase 1 family
  • 數(shù)據(jù)庫鏈接：

    UniGene: Rn.32104