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  4. Rat Tenascin-C(TN-C) ELISA Kit

Rat Tenascin-C(TN-C) ELISA Kit

  • 中文名稱：
    大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13377r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯(lián)免疫試劑盒（CSB-E13377r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TN-C含量。TN - C是當(dāng)前靶點(diǎn)。它在細(xì)胞外基質(zhì)中發(fā)揮重要作用，與細(xì)胞黏附、遷移等過(guò)程相關(guān)。研究其機(jī)制聚焦于它和腫瘤微環(huán)境、腫瘤細(xì)胞生長(zhǎng)及轉(zhuǎn)移的關(guān)系，通過(guò)揭示TN - C作用機(jī)制或?yàn)槟[瘤等相關(guān)疾病的治療提供新靶點(diǎn)與思路。試劑盒檢測(cè)范圍為2.5 ng/mL-160 ng/mL，科研人員可將其應(yīng)用于心血管疾病模型、肝臟纖維化研究或創(chuàng)傷修復(fù)機(jī)制探索，通過(guò)檢測(cè)不同病理狀態(tài)下TN - C的動(dòng)態(tài)表達(dá)，揭示其在細(xì)胞黏附、信號(hào)轉(zhuǎn)導(dǎo)中的功能，為靶向治療策略提供基礎(chǔ)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫：
    TN-C
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    2.5 ng/mL-160 ng/mL
  • 靈敏度：
    0.625 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 88-96    
    1:2 Average % 84    
    Range % 80-88    
    1:4 Average % 106    
    Range % 101-113    
    1:8 Average % 93    
    Range % 89-98    
  • 回收率：
    The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    160 2.417 2.502 2.460 2.301    
    80 2.102 2.173 2.138 1.979    
    40 1.700 1.615 1.658 1.499    
    20 1.085 1.049 1.067 0.908    
    10 0.671 0.684 0.678 0.519    
    5 0.424 0.437 0.431 0.272    
    2.5 0.293 0.281 0.287 0.128    
    0 0.162 0.156 0.159 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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