Rat Thioredoxin,Trx ELISA Kit
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中文名稱:大鼠硫氧化還原蛋白(Trx)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09729r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠硫氧化還原蛋白(Trx)酶聯(lián)免疫試劑盒(CSB-E09729r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TXN含量。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,適用于氧化應(yīng)激相關(guān)機(jī)制研究的科研場(chǎng)景;適用于研究大鼠疾病模型(如缺血再灌注損傷、神經(jīng)退行性病變等)中Trx的動(dòng)態(tài)變化,或評(píng)估抗氧化藥物干預(yù)效果,為氧化應(yīng)激相關(guān)分子機(jī)制探索提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:Txn ELISA Kit; Txn1 ELISA Kit; Thioredoxin ELISA Kit; Trx ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:3.12 ng/mL-200 ng/mL
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靈敏度:0.78 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Metabolism
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat Trx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 98 Range % 94-102 1:2 Average % 91 Range % 86-97 1:4 Average % 90 Range % 85-94 1:8 Average % 94 Range % 90-100 -
回收率:
The recovery of rat Trx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 97-109 EDTA plasma (n=4) 96 91-103 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 2.412 2.312 2.362 2.242 100 1.722 1.632 1.677 1.557 50 1.168 1.193 1.181 1.061 25 0.726 0.749 0.738 0.618 12.5 0.480 0.451 0.466 0.346 6.25 0.286 0.275 0.281 0.161 3.12 0.229 0.214 0.222 0.102 0 0.122 0.118 0.120 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Unveiling the Potential Effect of Nattokinase Against Radiation-Induced Acute Lung Injury in Rats NM Elbakary, RE Maarouf, AA Hassan,Natural Product Communications,2025
- Impact of zinc oxide nanoparticles on thioredoxin-interacting protein and asymmetric dimethylarginine as biochemical indicators of cardiovascular disorders in gamma-irradiated rats Abdel-Magied N, et al,Environmental Toxicology,2019
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
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基因功能參考文獻(xiàn):
- A delay in tumor formation and a lower cancerization rate was observed following the inhibition of Trx-1 with an irreversible inhibitor in the rat squamous cell carcinoma model. PMID: 29328386
- Data suggest that thioredoxin-1 (Trx1) modulates activity of soluble nitric oxide-sensitive guanylyl cyclase (GC1, subunits alpha and beta) through an association between Trx1 and GC1 via a mixed disulfide; Trx1 protects GC1 from desensitization. These studies were conducted, in part, using rat neonatal cardiomyocytes in primary culture. PMID: 28659344
- Trx1 exerts its neuroprotective effects by preventing oxidative stress in astrocytes via maintaining Prdx expression. PMID: 26846911
- Ginsenoside Rb1 shows a potent anti-oxidative effect on cultured PC12 cells by inducing Trx-1 expression. PMID: 26346161
- expression increased in cold adaptation PMID: 26327811
- Results suggest that Trx1 siRNA increases apoptotic stress-induced ASK1 activation and this represents further evidence that Trx1 is an endogenous anti-apoptotic molecule that diminishes focal cerebral ischemia/reperfusion injury PMID: 25541364
- both type 1 and type 2 diabetic rats demonstrated a significant increase in TxnIP mRNA and reduction in thioredoxin activity when compared to non-diabetic animals. PMID: 24925443
- Reduced form of Trx is required for cerebellar granule cell survival. PMID: 25055978
- Mycophenolic acid reduces the expression of Trx1 at the protein and gene levels, leading to the apoptosis of insulin-secreting cells via the MKK4/7-JNK pathway. PMID: 23846223
- Data indicate that Trx-1 is a therapeutic candidate for protecting against formaldehyde-induced neurotoxicity. PMID: 22342837
- the wave-like oscillations of Trx-1 expression during hypobaric hypoxia preconditioning was described in hypothalamus. PMID: 22165200
- Data suggest that Trx1 decreases in optic nerves and isolated retinal ganglion cells (RGC) after exposure to tumor necrosis factor-alpha in models of optic nerve degeneration with subsequent loss of RGC; 17beta-estradiol regulates expression of Trx1. PMID: 21586560
- Data provide implication of the Trx1/Txnip system in hepatic I/R injuries. PMID: 20709139
- respiration- and thioredoxin-dependent ROS removal as a potentially important mitochondrial function that may contribute to physiological and pathological processes in the brain PMID: 20558743
- Thioredoxin may mediate the neuroprotection conferred by estradiol against iron-induced brain injury in vivo. PMID: 19940280
- vitamin D(3)-up-regulated protein-1/thioredoxin axis has an important role in the preservation of cellular viability PMID: 12011048
- Activation of thioredoxin participates in the development of pressure-overload cardiac hypertrophy, demonstrating the dual function of thioredoxin as both an antioxidant and a signaling protein. PMID: 15123525
- reduced thioredoxin activity through interaction with Txnip is an important mechanism for vascular oxidative stress in diabetes mellitus PMID: 15128745
- AlphaAR-stimulated hypertrophic signaling in adult rat ventricular myocytes is mediated via a TRX1-sensitive posttranslational oxidative modification of thiols on Ras. PMID: 15723974
- during thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase, an intersubunit disulfide bond serves as a redox switch for activation PMID: 17130129
- Our findings indicate that cholestasis is associated with significant mutual and interrelated changes between circulating and hepatic thioredoxin and nitrosothiols. PMID: 17256724
- AngII induces thioredoxin in neuronal cultures. Thioredoxin then negatively regulates AngII stimulated neuronal activity via modulation of delayed rectifier K+ currents. PMID: 17360810
- Diabetes impairs exercise training-associated thioredoxin response and glutathione status in rat brain. PMID: 19074570
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亞細(xì)胞定位:Nucleus. Cytoplasm. Secreted.
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蛋白家族:Thioredoxin family
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數(shù)據(jù)庫(kù)鏈接:
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