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  4. Rat Thyroglobulin,TG ELISA Kit

Rat Thyroglobulin,TG ELISA Kit

  • 中文名稱：
    大鼠甲狀腺球蛋白(TG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08240r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠甲狀腺球蛋白(TG)酶聯(lián)免疫試劑盒（CSB-E08240r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TG含量。大鼠甲狀腺球蛋白（TG）是甲狀腺濾泡細胞合成的糖蛋白，參與甲狀腺激素的合成和儲存。其負反饋調(diào)節(jié)機制涉及抑制甲狀腺激素合成基因表達，與促甲狀腺激素（TSH）共同調(diào)控甲狀腺功能，對甲狀腺癌的診斷和預(yù)后評估具有重要意義。試劑盒檢測范圍為10 ng/mL-160 ng/mL，適用于科研領(lǐng)域?qū)谞钕俟δ芗跋嚓P(guān)疾病的機制研究；適用于甲狀腺相關(guān)藥物的作用評估、環(huán)境毒素對甲狀腺影響的機制研究，以及甲狀腺疾病動物模型中病理進程的動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    TgThyroglobulin ELISA kit; Tg ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue Homogenates
  • 檢測范圍：
    10 ng/mL-160 ng/mL
  • 靈敏度：
    10 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-102
    1:2 Average % 92
    Range % 86-99
    1:4 Average % 95
    Range % 87-105
    1:8 Average % 90
    Range % 83-102
  • 回收率：
    The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 89-100
    EDTA plasma (n=4) 95 88-103
  • 標(biāo)準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    10 2.553 2.568 2.561
    20 1.748 1.763 1.756
    40 0.885 0.875 0.880
    80 0.515 0.511 0.513
    160 0.285 0.287 0.286
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
  • 相關(guān)疾病：
    Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 組織特異性：
    Specifically expressed in the thyroid gland.
  • 數(shù)據(jù)庫鏈接：