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  4. Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

  • 中文名稱：
    大鼠組織因子途徑抑制物(TFPI)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08261r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠組織因子途徑抑制物(TFPI)酶聯(lián)免疫試劑盒（CSB-E08261r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TFPI含量。TFPI即組織因子途徑抑制物，是一種天然存在的抗凝蛋白。它能抑制組織因子（TF）介導(dǎo)的凝血途徑，通過與因子Xa和TF - 因子VIIa復(fù)合物結(jié)合，發(fā)揮抗凝作用。其在調(diào)節(jié)凝血平衡中起關(guān)鍵作用，對(duì)其研究有助于開發(fā)新型抗凝藥物等。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，為研究凝血機(jī)制、心血管疾病模型、藥物干預(yù)實(shí)驗(yàn)及炎癥相關(guān)研究提供可靠工具，特別適用于評(píng)估凝血相關(guān)因子調(diào)控、病理模型建立及治療性化合物篩選等科研場(chǎng)景，所有檢測(cè)結(jié)果僅用于科學(xué)研究目的。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-96  
    1:2 Average % 89  
    Range % 84-95  
    1:4 Average % 93  
    Range % 89-97  
    1:8 Average % 90  
    Range % 85-94  
  • 回收率：
    The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-107  
    EDTA plasma (n=4) 92 88-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    500 2.596 2.474 2.535 2.424  
    250 1.978 1.902 1.940 1.829  
    125 1.172 1.096 1.134 1.023  
    62.5 0.687 0.702 0.695 0.584  
    31.2 0.401 0.424 0.413 0.302  
    15.6 0.282 0.291 0.287 0.176  
    7.8 0.182 0.175 0.179 0.068  
    0 0.113 0.108 0.111    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 基因功能參考文獻(xiàn)：
    1. TFPI expression is induced in cardiomyocytes, but not in endothelial cells, by IL-1beta. PMID: 11816723
    2. Rapid atrial pacing acutely downregulated the gene expression of thrombomodulin and TFPI in rat atrial endocardium, thereby inducing local coagulation imbalance on the internal surface of the atrial cavity PMID: 14610015
    3. TFPI levels decreased significantly at 24 hours in burn, septic injury, and burn+septic injury groups, followed by incomplete rebound recovery at 72 hours PMID: 15497025
    4. This is the first report showing that direct inhibition of TF-mediated coagulation activation abrogates bleomycin-induced pulmonary fibrosis. PMID: 16338226
    5. analysis of tissue factor changes and the effects of tissue factor pathway inhibitor on transient focal cerebral ischemia in rats PMID: 18067952
    6. The results demonstrated that overexpression of TFPI gene might induce VSMC apoptosis in vitro through the mitochondrial pathway; meanwhile, IAP-1 expression is decreased. PMID: 18368493

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Most abundant in heart, lung, kidney, and aortic endothelial cells.
  • 數(shù)據(jù)庫(kù)鏈接：