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  4. Rat Transforming Growth factor β2,TGF-β2 ELISA kit

Rat Transforming Growth factor β2,TGF-β2 ELISA kit

  • 中文名稱：
    大鼠轉(zhuǎn)化生長(zhǎng)因子β2(TGF-β2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12111r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠轉(zhuǎn)化生長(zhǎng)因子β2(TGF-β2)酶聯(lián)免疫試劑盒（CSB-E12111r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TGFB2含量。TGFB2屬于轉(zhuǎn)化生長(zhǎng)因子β超家族成員，在胚胎發(fā)育、組織修復(fù)、免疫調(diào)節(jié)等生理過(guò)程中發(fā)揮關(guān)鍵作用。研究機(jī)制方面，它主要通過(guò)與相應(yīng)受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞增殖、分化和遷移，其異常表達(dá)與多種疾病如腫瘤、纖維化等相關(guān)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，可用于探索炎癥反應(yīng)、器官纖維化、胚胎發(fā)育或腫瘤發(fā)生等領(lǐng)域的分子機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 87  
    Range % 82-90  
    1:8 Average % 95  
    Range % 91-99  
  • 回收率：
    The recovery of rat TGF-β2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 83 81-85  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.869 2.897 2.883 2.691  
    500 2.231 2.267 2.249 2.057  
    250 1.544 1.598 1.571 1.379  
    125 0.969 0.987 0.978 0.786  
    62.5 0.638 0.609 0.624 0.432  
    31.2 0.452 0.472 0.462 0.270  
    15.6 0.307 0.321 0.314 0.122  
    0 0.191 0.193 0.192    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
  • 基因功能參考文獻(xiàn)：
    1. VEGF and TGFB2 are upregulated in femoral bone during fracture healing following implantation with coated titanium screws. PMID: 28361415
    2. Diminished collagen synthesis in folliculostellate cell-deficient cell aggregates was partially recovered by TGFbeta2. TGFbeta2-mediated folliculostellate cell-pericyte interaction appears to be essential for collagen synthesis in anterior pituitary. PMID: 26957638
    3. miR-378a expression is associated with its methylation status in TGF-beta1-treated cells, and epigenetically-regulated miR-378a inhibits TGF-beta1-induced hepatic stellate cells activation, at least in part, via TGF-beta2. PMID: 27855367
    4. Hepatic TGFB2 gene expression was up-regulated in liver of bile duct ligation model of liver fibrosis. PMID: 27049496
    5. These study results suggest that HR controls Tgf-b2 expression via regulation of miR-31, thus causing abnormal hair cycle in Hr(Hp)/Hr(Hp) mice. PMID: 25639665
    6. Data suggest that Muller cells release TGF-beta2, inhibiting the proliferation of retinal endothelial cells via activation of Smad2/Smad3 and attenuation of ERK signaling PMID: 24838936
    7. level of TGFbeta2 in rat colonic neoplasms PMID: 12209600
    8. Tgfb2 exhibited dynamic changes in expression in experimental glomerulonephritis. PMID: 12545247
    9. colocalizes with the trans-Golgi network marker TGN38 and a marker molecule for secretory granules, chromogranin B in PC12 cells and hippocampal neurons; a substantial portion of TGF-beta2 is secreted by the regulated secretory pathway in these cells PMID: 12595240
    10. TGF-beta 2 may play a physiological role in germ cell proliferation/differentiation and Sertoli cell function. PMID: 12646048
    11. role in cranial suture fusion PMID: 12733949
    12. Tgfb2 exrpession increased in infarcted myocardium after propranolol treatment. PMID: 14575314
    13. Analysis of Tgf-beta2 expression patterns in acute and progressive glomerulonephritis models. PMID: 15122060
    14. TGF-beta2 mRNA and protein expressions in the cerebral cortex of the H-Tx rat, a model of congenital hydrocephalus. PMID: 15375625
    15. TGF-beta2 plays important role in pancreatic morphogenesis and administration of excess all-trans retinoic acid before neurulation inhibit TGF-beta2 expression disrupted pancreatic morphogenesis particularly Langerhans islets. PMID: 16479313
    16. differential processing of TGF-beta1 and -beta 2 may be part of the mechanism that generates isoform-specific functions of the TGF-betas PMID: 17516499
    17. expression and signaling of TGFbeta2 and distribution of extracellular matrix proteins: laminin, fibronectin, collagens in the parotid gland of control and diabetic rats. PMID: 17574405
    18. novel role for TGF-beta2 in protection from unloading-induced apoptosis in vivo PMID: 18378961
    19. TGF-beta1 and TGF-beta 2 mRNA, but not TGF-beta3, is increased at 4 and 12 weeks in experimental diabetic peripheral neuropathy. PMID: 18406405
    20. immunolocalization of transforming growth factor beta (TGF-beta2) in rat thymic stromal cells and thymocytes: TGF-beta2 may play an important role in the organization or development of thymocytes in the late stages of thymopoiesis PMID: 18554691
    21. These results show that elevated expression of TSP-1 and TGF-beta can be detected in the injured segment of the spinal cord 12 and 24 h after injury. Thus, TSP-1 and TGF-beta may have a role in SCI. PMID: 19342245
    22. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-beta1 expression. PMID: 19434765
    23. TGF-beta2 is an essential neuroprotective factor released by microglia in its culture medium that, to be fully effective, requires the concomitant presence of other factor(s) of low molecular weight. PMID: 19457129
    24. TGF-beta2 and TGF-beta3 induced a dose-dependant increase of apoptosis in cultured stromal cells. PMID: 19656380

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  • 亞細(xì)胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 組織特異性：
    [Isoform TGF-beta2B]: Expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine.
  • 數(shù)據(jù)庫(kù)鏈接：