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  4. Rat Translationally-controlled tumor protein(TPT1) ELISA kit

Rat Translationally-controlled tumor protein(TPT1) ELISA kit

  • 中文名稱：
    大鼠翻譯調(diào)節(jié)腫瘤蛋白(TPT1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL024134RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠翻譯調(diào)節(jié)腫瘤蛋白(TPT1)酶聯(lián)免疫試劑盒（CSB-EL024134RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TPT1含量。TPT1是一個重要靶點(diǎn)。它與細(xì)胞內(nèi)多種生理過程相關(guān)，在腫瘤發(fā)生、發(fā)展等方面可能發(fā)揮作用。研究機(jī)制主要聚焦于其在細(xì)胞增殖、凋亡調(diào)控通路中的作用，探索其作為腫瘤診斷標(biāo)志物和治療靶點(diǎn)的可能，為相關(guān)疾病治療提供新思路。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于腫瘤生物學(xué)研究中的蛋白質(zhì)表達(dá)分析、藥物干預(yù)模型評價以及細(xì)胞應(yīng)激狀態(tài)下TPT1動態(tài)監(jiān)測等科研場景，為探索腫瘤發(fā)生機(jī)制及篩選潛在治療靶點(diǎn)提供精準(zhǔn)的體外檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; Lens epithelial protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.75 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-88
    1:2Average %94
    Range %90-98
    1:4Average %98
    Range %94-104
    1:8Average %95
    Range %88-99
  • 回收率：
    The recovery of rat TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9889-104
    EDTA plasma (n=4)9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    25002.047 2.134 2.091 1.959
    12501.384 1.483 1.434 1.302
    6250.854 0.836 0.845 0.713
    312.50.517 0.524 0.521 0.389
    1560.337 0.346 0.342 0.210
    780.257 0.248 0.253 0.121
    390.195 0.201 0.198 0.066
    00.134 0.129 0.132 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Involved in calcium binding and microtubule stabilization.
  • 基因功能參考文獻(xiàn)：
    1. Tumor protein translationally-controlled -induced dopamine release from neuronal PC12 cells was modulated by a Ca(2+)-independent mechanism that involved PLA in the process, suggesting the regulatory role of TCTP in the neuronal functions. PMID: 27783042
    2. After acute spinal cord injury, TCTP in astrocytes gradually increased, peaking at day 3 and returning to basal levels by day 14. TCTP knockdown reduced astrocyte migration and proliferation. PMID: 26266488
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    TCTP family
  • 數(shù)據(jù)庫鏈接：