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  4. Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

  • 中文名稱：
    大鼠肌鈣蛋白I，骨骼肌慢肌(TNNI1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL024011RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠肌鈣蛋白I，骨骼肌慢肌(TNNI1)酶聯(lián)免疫試劑盒（CSB-EL024011RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNNI1含量。TNNI1是心肌肌鈣蛋白I的慢骨骼肌亞型，主要表達(dá)于慢肌纖維。作為心肌重要調(diào)節(jié)蛋白，它參與肌肉收縮舒張的調(diào)控。當(dāng)前研究聚焦其在心肌病等疾病中表達(dá)變化及機(jī)制，以探索其作為潛在診斷標(biāo)志物和治療靶點(diǎn)的可能。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，本試劑盒適用于多種科研場(chǎng)景，如骨骼肌損傷模型的生物標(biāo)志物分析、肌肉代謝相關(guān)研究、運(yùn)動(dòng)醫(yī)學(xué)中肌纖維類型轉(zhuǎn)換機(jī)制探索，以及藥理學(xué)實(shí)驗(yàn)中藥物對(duì)慢肌功能影響的評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tnni1 ELISA Kit; Troponin I ELISA Kit; slow skeletal muscle ELISA Kit; Troponin I ELISA Kit; slow-twitch isoform ELISA Kit
  • 縮寫：
    TNNI1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 84    
    Range % 80-88    
    1:2 Average % 90    
    Range % 86-94    
    1:4 Average % 95    
    Range % 90-100    
    1:8 Average % 92    
    Range % 88-96    
  • 回收率：
    The recovery of rat TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 88 84-92    
    EDTA plasma (n=4) 103 99-108    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.526 2.423 2.475 2.289    
    500 1.875 1.756 1.816 1.630    
    250 1.212 1.159 1.186 1.000    
    125 0.784 0.811 0.798 0.612    
    62.5 0.500 0.490 0.495 0.309    
    31.2 0.347 0.362 0.355 0.169    
    15.6 0.291 0.274 0.283 0.097    
    0 0.183 0.188 0.186 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻(xiàn)：
    1. these results suggest that cTnI(R146G) and cTnI(R21C) blunt PKA modulation of activation and relaxation kinetics by prohibiting cardiac-specific N-terminal interaction with the cTnI inhibitory peptide. PMID: 26391394
    2. troponin I phosphorylation has a role in protein kinase C-mediated enhanced contractile performance of myocytes PMID: 12815045
    3. A novel role is described for cardiac TnI PKA phosphorylation in the rate-dependent enhancement of systolic & diastolic function in vivo & afterload sensitivity of relaxation. This has implications for cardiac failure. PMID: 14726477
    4. MuRF1 functions as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism PMID: 15601779
    5. TnI-isoform and titin-isoform switching and corresponding functional changes during heart development are not initiated by birth but are genetically programmed PMID: 16679402
    6. The goal here was to identify domain clusters and residues that confer troponin I isoform-specific myofilament Ca2+ and pH sensitivities of contraction. PMID: 17602701
    7. This study highlights cellular contractile defects by cardiomyopathy mutant cTnIs that are allele and species dependent. PMID: 18423659
    8. Study identifies tyrosine phosphorylation as a novel protein kinase Cdelta (PKCdelta) activation mechanism that modifies PKCdelta-dependent phosphorylation of cardiac troponin I. PMID: 18550549
    9. The negative inotropic effect of HIV gp120 is mediated by a novel signaling pathway that begins with binding to a cardiac myocyte CXCR4 receptor, followed by phosphorylation of both p38 MAP kinase and Troponin I. PMID: 18850076
    10. observed higher relative level of natural antisense RNA in neonatal cardiac fibroblasts compared to neonatal cardiomyocytes, pointing to a cell type-specific expression of sense and antisense RNA PMID: 19184367
    11. These findings suggest that altered TnI function, due to changes in the distribution of phosphorylated sites, is an early contributor to reduced contractility of the heart. PMID: 19507043

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  • 蛋白家族：
    Troponin I family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:29388

    STRING: 10116.ENSRNOP00000012096

    UniGene: Rn.4035