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  4. Rat Tryptophan 5-hydroxylase 2(TPH2) ELISA kit

Rat Tryptophan 5-hydroxylase 2(TPH2) ELISA kit

  • 中文名稱：
    大鼠色氨酸5羥化酶2(TPH2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL024101RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠色氨酸5羥化酶2(TPH2)酶聯(lián)免疫試劑盒（CSB-EL024101RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TPH2含量。TPH2即色氨酸羥化酶 2，是合成腦內(nèi)血清素的限速酶。其功能與情緒、認知等多種神經(jīng)活動緊密相關(guān)。研究聚焦于其在抑郁癥等精神疾病發(fā)病機制中的作用，探索通過調(diào)節(jié) TPH2 活性來干預血清素合成，為治療此類疾病提供新思路。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于神經(jīng)科學、精神疾病模型研究或藥物干預實驗中TPH2的動態(tài)監(jiān)測，例如探究抑郁癥、焦慮癥等疾病模型中5-HT系統(tǒng)的調(diào)控機制，或評估神經(jīng)活性藥物對TPH2表達的影響本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tph2 ELISA Kit; Ntph ELISA Kit; Tryptophan 5-hydroxylase 2 ELISA Kit; EC 1.14.16.4 ELISA Kit; Neuronal tryptophan hydroxylase ELISA Kit; Tryptophan 5-monooxygenase 2 ELISA Kit
  • 縮寫：
    TPH2
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 96    
    Range % 93-102    
    1:2 Average % 94    
    Range % 87-98    
    1:4 Average % 102    
    Range % 96-105    
    1:8 Average % 91    
    Range % 87-95    
  • 回收率：
    The recovery of rat TPH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 89-96    
    EDTA plasma (n=4) 97 90-102    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.123 2.262 2.193 2.092    
    1000 1.495 1.585 1.540 1.439    
    500 1.012 1.044 1.028 0.927    
    250 0.664 0.677 0.671 0.570    
    125 0.442 0.451 0.447 0.346    
    62.5 0.276 0.289 0.283 0.182    
    31.25 0.191 0.195 0.193 0.092    
    0 0.102 0.100 0.101 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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