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  4. Rat Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ ELISA KIT

Rat Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ ELISA KIT

  • 中文名稱：
    大鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07381r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)酶聯(lián)免疫試劑盒（CSB-E07381r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNFsR1含量。TNFR1（Tumor Necrosis Factor Receptor 1），又稱TNF-R55或CD120a，是一種與腫瘤壞死因子α（TNF-α）相互作用的受體蛋白，在多種生理和病理過程中發(fā)揮關(guān)鍵作用。其通過與TNF-α結(jié)合，觸發(fā)信號傳導(dǎo)，參與細(xì)胞凋亡、炎癥反應(yīng)和免疫調(diào)節(jié)等過程。研究顯示，TNFR1在自身免疫疾病、炎癥性疾病和癌癥治療中具有潛在應(yīng)用價值。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，炎癥機(jī)制研究、免疫相關(guān)疾病動物模型評估、藥物干預(yù)效果分析、TNF/TNFR 信號通路調(diào)控、病理模型建立及生物標(biāo)志物篩選等科研場景，適合需要多類型樣本兼容的實驗室使用本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tnfrsf1a; Tnfr-1; Tnfr1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD antigen CD120a
  • 縮寫：
    TNFsR-
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 100-110
    1:2 Average % 92
    Range % 88-96
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 90
    Range % 85-94
  • 回收率：
    The recovery of rat TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 82-93
    EDTA plasma (n=4) 95 90-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.107 2.221 2.164 1.987
    500 1.562 1.499 1.531 1.354
    250 0.952 1.003 0.978 0.801
    125 0.573 0.558 0.566 0.389
    62.5 0.395 0.412 0.404 0.227
    31.2 0.284 0.275 0.280 0.103
    15.6 0.229 0.234 0.232 0.055
    0 0.181 0.173 0.177 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
  • 基因功能參考文獻(xiàn)：
    1. Activation of TNF-alpha receptor 1 in the subfornical organ (SFO) contributes to sympathetic excitation in heart failure rats by increasing inflammation and renin-angiotensin system activity PMID: 28710070
    2. Resveratrol restores the antinociceptive effect of morphine by reversing morphine infusion-induced spinal cord neuroinflammation and increase in TNFR1 expression. The reversal of the morphine-induced increase in TNFR1 expression by resveratrol is partially due to reversal of the morphine infusion-induced increase in HDAC1 expression. PMID: 26078221
    3. TNF plays an inhibitory role in modulating myocardial SDF-1 production and blockade of TNF signaling by ablation of TNFR1 and TNFR2 genes increased SDF-1 expression in the heart. These data expand on TNF signaling-initiated mechanisms in myocardium, which may lend a more complete understanding of SDF-1 and TNFR-derived actions in hopes of advancing ischemic heart injury treatments. PMID: 27979472
    4. miR-29a might promote the apoptosis of pancreatic acinar cells via up-regulating the expression of its target gene TNFRSF1A. PMID: 27239114
    5. TNFR1 and TNFR2 regulate voltage-gated sodium channels in rat dorsal root ganglia PMID: 26504355
    6. Co-culture with bone marrow stromal cells protects PC12 neurons from TNF-alpha-induced apoptosis by inhibiting the TNFR1/caspase 8 signaling pathway. PMID: 25738414
    7. Data indicate that combination of tumor necrosis factor (TNF) and TNF receptor (TNFR) on the cell membrane activated two downstream signal pathways. PMID: 23134577
    8. Blocking TNF-alpha binding to TNFR1 by CAY10500 intravenously slightly mitigates pulmonary inflammation, but cannot improve the pulmonary function, indicating the limited role of TNFR1 pathway in circulating inflammatory cell in ECC-induced ALI. PMID: 24599692
    9. antisense to IL-6 receptor subunit gp130 (gp130), but not to tumor necrosis factor receptor type 1 (TNFR1), inhibited hyperalgesia in NLB rats. However, antisense against either gp130 or TNFR1 inhibited sound stress-induced enhancement of hyperalgesia. PMID: 23706525
    10. Ultra-low dose naloxone enhances the antihyperalgesia and antiallodynia effects of morphine in rats with partial sciatic nerve transection, possibly by reducing TNF-alpha and TNFR1 expression in the spinal dorsal horn. PMID: 24257399
    11. Ozone suppresses joint swelling in rheumatoid arthrtis by down-regulating synovial TNF-alpha and TNF-R2 and up-regulating TNF-R1 in the joint. PMID: 23052485
    12. exDCs transduced to overexpress soluble tumor necrosis factor (TNF) receptor1 (sTNFR1) produce functional cargo that is secreted and that blocks TNF-alpha bioavailability in vitro. PMID: 23756688
    13. The microglial TNFalpha receptor p55 pathway plays a more important role than the TNFalpha-p75 pathway in the pathogenesis of sciatic nerve injury pain. PMID: 22652595
    14. Fas and TNFR1 are expressed in the CA1 and CA3 regions after global ischemia/reperfusion in rats. Bcl-2 overexpression decreases the expression of Fas and TNFR1 and cell apoptosis after global IR. PMID: 21868309
    15. Tnfrsf1a, Birc2 and Birc3 of Schwann cells, not originally regarded as XIAP, were mainly responsible for the inflammation-mediated anti-apoptosis of peripheral nerves. PMID: 23028454
    16. Intra-articular injection of ozone can attenuate synovitis in rats with rheumatoid arthritis, which may involve the inhibition of TNF-alpha and TNFR II activity and enhancement of TNFR I activity in the synovium. PMID: 21690068
    17. The moxibustion therapies could reduce intestinal inflammation and restore intestinal epithelium barrier disruption in CD, which might be due to down-regulating TNF-alpha, TNFR1, and TNFR2 in intestinal mucosa and improving intestinal epithelium morphology. PMID: 21359923
    18. Moxibustion may inhibit colonic epithelial cell apoptosis by reducing the high expression of TNF-alpha and TNFR1 to protect the defective colonic epithelial barrier in Crohn disease model rats. PMID: 22531889
    19. Data show that cerebral ischemia and organ culture induce expression of TNF-alpha and its receptors TNFR1 and 2 in the walls of cerebral arteries and that upregulation is transcriptionally regulated via the MEK/ERK pathway. PMID: 21871121
    20. Up-regulation of p55 in dorsal root ganglia neurons may be involved in the sensory transmission from facet joint injury. PMID: 17468886
    21. Data show that TNFR1 and TNFR2 are constitutively expressed in Kupffer cells and their expression is strongly increased by somatostatin. PMID: 21692635
    22. Vagal nerve stimulation can rectify ischaemia-induced cardiac dysfunction partly via inhibition of a TNF-alpha-mediated signalling pathway. PMID: 21362018
    23. doxorubicin increases diaphragm sensitivity to TNF by upregulating TNFR1, thereby causing respiratory muscle weakness PMID: 21097524
    24. Cultured hypothalamic neurons express IL-1RI and that LPS + IFN-gamma increases this expression. PMID: 20619468
    25. Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780
    26. Data show that exercise training enhanced the ability of adipocytes to secrete TNF-alpha with reduced secretion of type 1 soluble TNF receptor, and provoked the greater expressions of mTNFR1 in adipocyte crude membrane. PMID: 12052444
    27. ASK1 may be involved in the mechanism of seizure-induced neuronal death downstream of a TNFR1 death-signalling complex. PMID: 12786973
    28. Both TNF receptors were detected, with TNF-R1 being prominent on bronchial epithelial cells and endothelial cells, and TNF-R2 being expressed by nearly all cell types PMID: 12849708
    29. Up-regulation of tumor necrosis factor receptor I in tumor-bearing rats is associated with cancer-related cachexia PMID: 12851692
    30. The expression of TNFR1 and TNFR2 after spinal cord injury may contribute to posttraumatic inflammatory responses of TNF-alpha PMID: 14552870
    31. In conclusion, protein deficiency in endotoxic rats increases the expression of TNFR-I and TNFR-II in all organs studied and TNF-alpha in selected ones. PMID: 14625205
    32. Increased TNfr1 receptor immunoreactivity in axotomized neurons may play a pivotal role in the connection between the occurrence of the injury and the initiation of apoptotic processes resulting in elimination of damaged neurons. PMID: 15033431
    33. At all spinal levels, TNFR1 receptor is constitutively expressed on cells and afferent fibers within the dorsal root ganglia, afferent fibers of the dorsal root, dorsal root entry zone and within lamina I and II of the dorsal horn. PMID: 15464762
    34. We provide evidence that non-neuronally synthesized TNFalpha may directly act on primary afferent neurons via TNFR1 but not TNFR2. PMID: 15509749
    35. It is concluded that the shedding of TNFR1 receptor may represent an important post-traumatic physiological response aimed at reducing the proapoptotic effect of TNF-alpha. PMID: 16083358
    36. Upregulation of TNF-alpha and TNFR1 in dorsal root ganglia and spinal dorsal horn is essential for initiation but not for maintenance of neuropathic pain induced by L5 ventral root transection. PMID: 16675114
    37. Cellular distribution of TNFR1 in the dorsal root ganglia may reflect different pathways by which TNF-alpha effect on the primary sensory neurons can be mediated following nerve injury. PMID: 16705482
    38. EGFR, FGFR and TNFR1 mRNA expression was equal or lower than in resting liver, HGFR expression after I/R was stronger than in the control. PMID: 16804967
    39. cyclic tensile strain inhibited the IL-1beta-stimulated synthesis of TNF-alpha, TNFR2, and iNOS PMID: 17049356
    40. study of TNF-alpha and TnfrR1 protein synthesis and expression patterns in sciatic nerve of controls and rats under systemic challenge with lipopolysaccharide. PMID: 17873366
    41. These results suggest that an autocrine loop involving TNF-alpha, TNFR1 and TNFR2 contributes to the production of beta-1,4-GalT I and V mRNA in response to inflammation. PMID: 17917074
    42. Data show that cultured Schwann cells express both tumor necrosis factor receptor 1 (TNFR1) and TNFR2, and that activation of these receptors by TNF-alpha promotes expression of TNF-alpha. PMID: 18205044
    43. these data suggest that tumor necrosis factor receptor 1 induces induces interleukin-6 upregulation and neuropathic pain through NF-kappaB, but not p38 MAPK activation in the spinal cord PMID: 18938092
    44. results demonstrate the existence of TNFR1 and TNFR2 in rat corpus luteum and suggest the involvement of TNF-alpha in rat CL regression following parturition PMID: 18990246
    45. These data suggest that TNFRI-mediated signals in the radioresistant tissues contribute to disease progression, whereas TNFRI-mediated signals in the radiosensitive tissues can contribute to protection from disease. PMID: 19180511

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：