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  4. Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

  • 中文名稱：
    大鼠尿苷二磷酸葡萄糖醛酸轉(zhuǎn)移酶1(UGT1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13845r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠尿苷二磷酸葡萄糖醛酸轉(zhuǎn)移酶1(UGT1)酶聯(lián)免疫試劑盒（CSB-E13845r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的UGT1A1含量。UGT1A1是重要的藥物代謝酶基因，在人體肝臟中表達(dá)。它參與多種內(nèi)源性和外源性物質(zhì)的葡萄糖醛酸化代謝反應(yīng)。研究其機(jī)制有助于了解藥物代謝過程、藥物相互作用，對(duì)評(píng)估藥物療效和不良反應(yīng)、實(shí)現(xiàn)精準(zhǔn)用藥有重要意義。試劑盒檢測(cè)范圍為31.2 pg/mL-2000 pg/mL，該產(chǎn)品適用于藥理學(xué)研究、肝臟功能評(píng)估、代謝調(diào)控機(jī)制探索等領(lǐng)域，尤其適合用于研究藥物干預(yù)、毒素暴露或病理狀態(tài)下UGT1的活性調(diào)控及組織分布特征，為毒理學(xué)、藥代動(dòng)力學(xué)及代謝性疾病研究提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ugt1a1; Ugt1; UDP-glucuronosyltransferase 1A1; UGT1A1; B1; UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.2 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat UGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-91
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 107
    Range % 103-110
    1:8 Average % 110
    Range % 104-114
  • 回收率：
    The recovery of rat UGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 91-99
    EDTA plasma (n=4) 103 98-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 1.941 2.014 1.978 1.875
    1000 1.503 1.498 1.501 1.398
    500 0.999 1.031 1.015 0.912
    250 0.610 0.631 0.621 0.518
    125 0.417 0.407 0.412 0.309
    62.5 0.235 0.228 0.232 0.129
    31.25 0.148 0.150 0.149 0.046
    0 0.101 0.104 0.103 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol. Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates. Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties. Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II. Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan.
  • 基因功能參考文獻(xiàn)：
    1. study indicated that Status Epilepticus altered the expression of brain Ugt1a1 and Ugt1a7, which could alter glucuronidation in the brain. PMID: 29131354
    2. UGT1A1 controls estrogen metabolism. PMID: 28479355
    3. Data indicate that the up-regulation of phase II detoxification in the DR group was confirmed by mRNA and protein expression levels of uridinediphospho-glucuronosyltransferase and glycine-N-acyltransferase in actual liver tissues. PMID: 23230277
    4. Both fipronil and fipronil sulfone treatments induced a 2.5-fold increase in Ugt1a1 and Sult1b1 messenger RNA (mRNA) expressions. PMID: 22447239
    5. UDP-glucuronosyltransferase (UDP-GT) activities towards p-nitrophenol were markedly increased in rats that consumed tea.Induction of UDP-glucuronosyltransferase activity by tea may involve the UDP-GT1 (UGT1A) gene complex of the UDP-GT multigene family PMID: 11983278
    6. These results indicate that the expression of extrahepatic UDP-glucuronosyltransferases UGT1a1 and UGT1a6 is isoform-specific and regulated differentially in tissues by the pituitary gland. PMID: 12153725
    7. Our findings show that the absence of UGT1 protein from Gunn rat hepatocytes is due to rapid degradation of the truncated UGT1 protein by the proteasome and elucidate the molecular basis underlying the deficiency in bilirubin glucuronidation. PMID: 12220528
    8. Developmental stage-specific switching of regulation of the rat UGT1 gene complex was found. PMID: 18938141
    9. Data suggest that thyroid hormones and vitamin A are co-regulators of UDP-glucuronosyltransferase (UGT) 1 expression, without affecting the UGT2 family PMID: 11997190

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  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    UDP-glycosyltransferase family
  • 數(shù)據(jù)庫(kù)鏈接：