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  4. Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

  • 中文名稱(chēng)：
    大鼠泛素羧基末端水解酶L1(UCHL1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL025541RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠泛素羧基末端水解酶L1(UCHL1)酶聯(lián)免疫試劑盒（CSB-EL025541RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的UCHL1含量。大鼠泛素羧基末端水解酶L1（UCHL1）在多種生理和病理過(guò)程中發(fā)揮重要作用，包括神經(jīng)元功能、細(xì)胞周期調(diào)控和泛素化過(guò)程。其表達(dá)與細(xì)胞狀態(tài)和代謝活性相關(guān)，并在多種疾病如神經(jīng)退行性疾病和炎癥反應(yīng)中扮演關(guān)鍵角色。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，該產(chǎn)品操作流程標(biāo)準(zhǔn)化，可在4.5小時(shí)內(nèi)完成檢測(cè)，為科研人員研究泛素 - 蛋白酶體系統(tǒng)功能、神經(jīng)退行性病變分子機(jī)制、藥物干預(yù)效果評(píng)估等提供可靠工具，尤其適用于體外模型構(gòu)建、疾病標(biāo)志物篩選及分子通路調(diào)控等基礎(chǔ)研究領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Uchl1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; UCH-L1 ELISA Kit; EC 3.4.19.12 ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit
  • 縮寫(xiě)：
    UCHL1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat UCHL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %84-93
    1:2Average %101
    Range %98-106
    1:4Average %89
    Range %85-93
    1:8Average %104
    Range %100-108
  • 回收率：
    The recovery of rat UCHL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-90
    EDTA plasma (n=4)9693-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.196 2.174 2.185 2.050
    251.618 1.635 1.627 1.492
    12.51.071 1.098 1.085 0.950
    6.250.682 0.654 0.668 0.533
    3.120.450 0.436 0.443 0.308
    1.560.356 0.368 0.362 0.227
    0.780.251 0.265 0.258 0.123
    00.134 0.136 0.135
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 基因功能參考文獻(xiàn)：
    1. UCH-L1 can inhibit TNF-alpha-induced vascular smooth muscle cell migration, and this kind of effect may be partially due to its suppression role in NF-kappaB activation. PMID: 30305579
    2. investigated temporal profile of astrocytic and neuronal injury markers after TBI; different mechanisms underlie clearance of UCH-L1 and GFAP in CSF and serum PMID: 25763798
    3. Potential of UCHL1 as biomarker for destruction of pancreatic beta cells. PMID: 25638021
    4. UCH-L1 is differently processed in neurons compared with clonal cell lines and that farnesylation does not account for the membrane association in neurons. PMID: 25326379
    5. The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis. PMID: 24090154
    6. in AD, Abeta may down-regulate UCH-L1 in the AD brain, which in turn impairs BDNF/TrkB-mediated retrograde signaling, compromising synaptic plasticity and neuronal survival. PMID: 23599427
    7. Restoring UCH-L1 activity by transduction of primary neurons with a UCH-L1 fusion protein protected neurons, while inhibition of UCH-L1 exacerbates hypoxic injury. PMID: 20933087
    8. PGP9.5 messenger was decreased in nitrofen-exposed animals on E18 (esophagus) or E15 (small bowel), and increased on E21 in the esophagus and E18 in small bowel. PMID: 21069350
    9. Zinc deficiency could induce downregulation of Uch-L1 expression. PMID: 19267071
    10. analysis of a possible mechanistic link between UCH-L1 modification by cyclopentenone prostaglandins and the etiology of neurodegeneration PMID: 20231490
    11. Complete loss of UCH-L1 immunoreactivity in orofacial skin nerve fibers in dithranol-treated animals. PMID: 17011532
    12. UCH-L1 is widely expressed in neurons in all brain regions analyzed, including dopamine neurons of the midbrain. In the periphery, UCH-L1 expression remains restricted to neurons in all organs examined. PMID: 17599367
    13. UCHL1 may partially attenuate vascular remodeling through inhibition of NF-kappaB activity. PMID: 17690318
    14. results demonstrated that zinc modulated the expression of ubiquitin C-terminal hydrolase L1 (Uch-L1) and cAMP-responsive element-binding protein (CREB) at the protein and/or transcription levels in hippocampal neurons PMID: 18616865

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  • 亞細(xì)胞定位：
    Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor.
  • 蛋白家族：
    Peptidase C12 family
  • 數(shù)據(jù)庫(kù)鏈接：