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  4. Rat Vascular endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit

Rat Vascular endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit

  • 中文名稱：
    大鼠血管內(nèi)皮細(xì)胞生長因子受體1(VEGFR-1/Flt1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07350r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血管內(nèi)皮細(xì)胞生長因子受體1(VEGFR-1/Flt1)酶聯(lián)免疫試劑盒（CSB-E07350r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VEGFR-1/Flt1含量。VEGFR-1/Flt1作為靶點，在血管生成等生理過程中起重要作用。其背景在于它是血管內(nèi)皮生長因子受體家族成員。研究機(jī)制方面，主要聚焦于其信號傳導(dǎo)通路，通過與配體結(jié)合傳遞信號，影響細(xì)胞的增殖、遷移等，對腫瘤血管生成等疾病研究有重要意義。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用各類血管相關(guān)疾病模型的機(jī)制研究，如腫瘤血管生成實驗、心血管病理分析或藥物干預(yù)后受體表達(dá)水平評估，特別適用于需要高靈敏度檢測血管內(nèi)皮細(xì)胞功能標(biāo)志物的基礎(chǔ)研究領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Flt1 ELISA Kit; Flt-1 ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-101  
    1:2 Average % 103  
    Range % 99-110  
    1:4 Average % 88  
    Range % 84-92  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of rat VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 84 80-88  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    100 2.621 2.557 2.589 2.412  
    50 2.005 1.946 1.976 1.799  
    25 1.286 1.332 1.309 1.132  
    12.5 0.893 0.859 0.876 0.699  
    6.25 0.514 0.521 0.518 0.341  
    3.12 0.397 0.408 0.403 0.226  
    1.56 0.260 0.252 0.256 0.079  
    0 0.179 0.174 0.177    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1.
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Endosome.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 數(shù)據(jù)庫鏈接：