Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血管活性腸肽(VIP)酶聯(lián)免疫試劑盒（CSB-E08355r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VIP含量。VIP即血管活性腸肽，具有廣泛生物學(xué)活性。它參與調(diào)節(jié)心血管、消化等系統(tǒng)功能。在研究機(jī)制上，VIP通過(guò)與特定受體結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路，影響多種生理和病理過(guò)程，在神經(jīng)保護(hù)、抗炎等方面展現(xiàn)潛在治療價(jià)值。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，適用于神經(jīng)生物學(xué)、消化系統(tǒng)疾病模型研究，可分析大鼠外周循環(huán)或組織局部VIP表達(dá)水平，為探究胃腸動(dòng)力障礙、炎癥調(diào)節(jié)機(jī)制及神經(jīng) - 免疫交互作用提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

VIP
Uniprot No.：

P32649 P01283
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	93-101
1:4	Average %	100
	Range %	97-103
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.