Rat activated coagulation factor X(FXa) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠活化凝血因子X(FXa)酶聯(lián)免疫試劑盒（CSB-EQ027251RA）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FXa含量。FXa即凝血因子Xa，是凝血級聯(lián)反應(yīng)中的關(guān)鍵靶點。它在凝血過程中處于內(nèi)、外源凝血途徑交匯點，激活后促使凝血酶原轉(zhuǎn)化為凝血酶。對其研究機(jī)制主要聚焦于開發(fā)抑制劑，以抑制凝血過程，用于血栓性疾病防治。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，支持實驗室開展凝血機(jī)制研究、抗凝藥物篩選、病理模型評估等方向?qū)嶒灒鄻颖炯嫒菪栽O(shè)計尤其適合系統(tǒng)性研究凝血因子在組織微環(huán)境中的動態(tài)變化，為探索血栓性疾病、術(shù)后凝血異常等課題提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FXa
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.