Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit
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中文名稱:大鼠α1微球蛋白/bikunin蛋白前體(AMBP)酶聯(lián)免疫試劑盒
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貨號:CSB-EL001654RA
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠α1微球蛋白/bikunin蛋白前體(AMBP)酶聯(lián)免疫試劑盒(CSB-EL001654RA)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AMBP含量。試劑盒檢測范圍為1.563 ng/mL-100 ng/mL,靈敏度為0.649 ng/mL。為科研領(lǐng)域研究AMBP在代謝性疾病模型、組織損傷修復機制或藥物干預效果評估中的動態(tài)變化提供可靠工具,尤其適用于動物實驗中的生物標志物探索及分子通路機制解析本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
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縮寫:AMBP
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1.563 ng/mL-100 ng/mL
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靈敏度:0.649 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 105 Range % 99-109 1:200 Average % 96 Range % 92-99 1:400 Average % 95 Range % 90-98 1:800 Average % 89 Range % 84-94 -
回收率:
The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-95 EDTA plasma (n=4) 99 99-104 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 100 0.116 0.124 0.120 50 0.231 0.245 0.238 25 0.478 0.498 0.488 12.5 1.019 1.025 1.022 6.25 1.426 1.367 1.397 3.125 2.159 2.168 2.164 1.563 2.466 2.544 2.505 0 2.845 2.654 2.750 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:AMBP(α1酸性糖蛋白)是一種由肝臟合成的糖蛋白,具有多種生物學功能,包括與免疫系統(tǒng)、炎癥反應(yīng)和病原體識別相關(guān)。最近的研究表明,AMBP在多種疾病中發(fā)揮著重要作用,例如腎臟疾病、肝病、癌癥、糖尿病、心血管疾病等。以下是AMBP的最新研究進展:腎臟疾?。篈MBP被認為是腎臟疾病的標志物之一,其水平與腎小球濾過率、尿蛋白和炎癥反應(yīng)相關(guān)。最近的研究還發(fā)現(xiàn),AMBP可以作為腎小球腎炎的標志物,有助于診斷和監(jiān)測該疾病的進展。癌癥:AMBP的水平在多種癌癥中明顯升高,包括肝癌、胃癌、肺癌等。最近的研究表明,AMBP可能參與了腫瘤的發(fā)生、發(fā)展和轉(zhuǎn)移,并且可以作為癌癥的潛在治療靶點。免疫系統(tǒng):AMBP可以通過多種方式參與免疫系統(tǒng)調(diào)節(jié),包括識別和清除病原體、調(diào)節(jié)T細胞和B細胞功能、參與炎癥反應(yīng)等。最近的研究表明,AMBP在免疫系統(tǒng)調(diào)節(jié)中的作用可能是通過與其他分子相互作用,如IgG、Toll樣受體(TLR)等。
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功能:Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.; Trypstatin is a trypsin inhibitor. It inhibits blood coagulation factor Xa and tryptase about 100-fold more rapidly than porcine pancreatic trypsin and chymase.
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亞細胞定位:Secreted.
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蛋白家族:Calycin superfamily, Lipocalin family
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組織特異性:Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. Trypstatin is present in mast cell gra
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