Rat apolipoprotein B-48 (ApoB48) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠載脂蛋白B-48(ApoB48)酶聯(lián)免疫試劑盒（CSB-EQ027557RA）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的ApoB48含量。ApoB48 是一種重要靶點。它主要在小腸合成，參與乳糜微粒組裝和分泌。研究發(fā)現(xiàn)其與脂質(zhì)代謝密切相關(guān)，異常升高可能導(dǎo)致血脂異常等問題。目前科研聚焦于通過抑制 ApoB48 生成或功能，調(diào)節(jié)脂質(zhì)代謝，為治療相關(guān)疾病提供新思路。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，適用于營養(yǎng)干預(yù)實驗、代謝性疾病模型研究、藥物對腸道脂質(zhì)吸收影響的評估等科研領(lǐng)域，為探索脂代謝調(diào)控機(jī)制及心血管疾病相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ApoB48
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.078 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ApoB48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	96
	Range %	92-102
1:400	Average %	95
	Range %	89-100
1:800	Average %	92
	Range %	89-96

回收率：

The recovery of rat ApoB48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	92	88-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.