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  4. Rat apolipoprotein B100 (Apo-B100) ELISA Kit

Rat apolipoprotein B100 (Apo-B100) ELISA Kit

  • 中文名稱：
    大鼠載脂蛋白B100(Apo-B100)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08102r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠載脂蛋白B100(Apo-B100)酶聯(lián)免疫試劑盒（CSB-E08102r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOB含量。APOB 作為重要靶點，它與脂質(zhì)代謝密切相關(guān)。APOB 蛋白是低密度脂蛋白等的關(guān)鍵組成部分，其表達異?？赡芤l(fā)高脂血癥等疾病。研究多聚焦于調(diào)控 APOB 表達或功能，以減少致動脈粥樣硬化脂蛋白的產(chǎn)生，為心血管疾病防治提供新策略。試劑盒檢測范圍為0.313 μg/mL-20 μg/mL，靈敏度為0.342 μg/mL。適用于實驗室開展脂代謝異常動物模型評估、肝臟脂蛋白合成機制研究以及心血管疾病相關(guān)生物標志物探索等科研項目。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Apob ELISA kit; Aa1064 ELISA kit; Ac1-060Apolipoprotein B-100 ELISA kit; Apo B-100) [Cleaved into: Apolipoprotein B-48 ELISA kit; Apo B-48)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.313 μg/mL-20 μg/mL
  • 靈敏度：
    0.342 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess. 

  • 線性度：

     To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 標準曲線：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:


My samples are hemolyzed....could it be any problem with this samples when using the kits?

A:
Thanks for your inquiry. Serious hemolysis is not suitable for testing. You need to re-collect the samples.
If it is slightly hemolyzed, the impact is small and the samples can be used.
The sensitivity of this kit is relatively good, but we still advice you to use dual-wavelength detection to eliminate the impact.

靶點詳情

  • 最新研究進展：
        APOB（Apolipoprotein B）是一種在人體內(nèi)主要由肝臟合成的大型蛋白質(zhì)，其主要功能是在低密度脂蛋白（LDL）的形成和代謝中發(fā)揮重要作用。最新研究表明，APOB可通過與非酒精性脂肪肝病（NAFLD）發(fā)生關(guān)聯(lián)，同時與心臟病、中風等疾病的發(fā)病風險密切相關(guān)。此外，研究人員還發(fā)現(xiàn)，在APOB的N端和C端區(qū)域存在多個功能區(qū)域，這些區(qū)域在肝臟合成APOB時發(fā)揮了不同的作用，對APOB的代謝和功能具有重要影響。
  • 功能：
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 基因功能參考文獻：
    1. enhanced VLDL TG secretion in the absence of hepatocyte ABCA1 is due to altered intracellular trafficking of apolipoprotein B (apoB), resulting in augmented TG addition to nascent VLDL. PMID: 28694219
    2. Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
    3. Coordinated up-regulation of Mttp, Apob, Hnf1a, Hnf4a, Fasn, Gpam and Dgat (1 and 2) gene expressions may contribute to the increase in circulating and liver concentrations of TAGs after lipectomy in an experimental rat model PMID: 26184122
    4. Overall, results further support the importance of Class I PI3K activity in IDAD, and suggest that loss of this activity is sufficient to cause resistance. PMID: 26616056
    5. KLHL12 plays a critical role in facilitating the endoplasmic reticulum exit and secretion of apoB100-VLDL particles, suggesting that KLHL12 modulation would influence plasma lipid levels. PMID: 24334870
    6. Report abnormal gene/protein expression and phosphorylation of multiple pathways related to inflammatory, insulin signaling and lipogenesis may be important underlying factors in VLDL-apoB100 particles overproduction observed in type 2 diabetes. PMID: 22546076
    7. Doxycycline induced apoA-IV expression in McA-RH7777 cells alters apoB and lipid secretion. PMID: 22257482
    8. JCR:LA-cp rats are used as a model of postprandial lipemia to explore chylomicron metabolism during the onset and development of insulin-resistance by showing impaired apolipoprotein b-48 blood levels. PMID: 16624317
    9. It was concluded that IL-6 favors secretion of apoB-containing lipoproteins by increasing availability of apoB through changes in apob gene transcription. PMID: 20651008
    10. A factor(s) other than microsomal triglyceride transfer protein mediates the formation of the phospholipid-rich primordial apoB:1000-containing initiation complex. PMID: 20181985
    11. the developmental regulation of apoB mRNA editing is an autonomous cytodifferentiation function of small intestine and involves Cdx1 PMID: 12493769
    12. pulse/chase protocols and subcellular fractionation showed apoB100 bulk lipidation, resulting in conversion of LDL/HDL to VLDL, can occur in the ER, but not in the Golgi, in McA RH7777 cells PMID: 12917397
    13. apoB100 lipoproteins exit the endoplasmic reticulum in COPII vesicles, but under conditions favorable for very low density lipoprotein formation final lipid loading occurs post-endoplasmic reticulum PMID: 12960170
    14. apoB mRNA editing is modulated at the level of assembly by alterations in the relative abundance of multiple related RNA-binding auxiliary proteins. PMID: 14570923
    15. increased FA delivery to the liver increases secretion of apoB; oleic acid -induced apoB secretion occurred via mechanisms other than by providing substrate for TG synthesis; and the route of delivery of FA is important for its effects on apoB secretion. PMID: 14970200
    16. Lipid peroxidation and oxidative stress regulate hepatic Apob degradation and VLDL production. PMID: 15124019
    17. the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation PMID: 15520448
    18. Hepatic Betaine-Homocysteine S-Methyltransferase expression was induced by feeding rats a Met (L-methionine)-restricted betaine-containing diet, and parameters of ApoB metabolism were evaluated. PMID: 16396637
    19. apolipoprotein B secretion, but not lipid, is upregulated by tumor necrosis factor-alpha in rat hepatocyte cultures in the absence of extracellular fatty acids PMID: 17405916
    20. the initial addition of phospholipids to apoB:1000 and initiation of apoB-containing lipoprotein assembly occur independently of MTP lipid transfer activity. PMID: 17690102
    21. apoB secretory control via PERPP/autophagosomes is likely a key component of normal and pathologic regulation of plasma apoB levels, as well as a means for remarkably late-stage quality control of a secreted protein PMID: 18391222
    22. Localization of microsomal triglyceride transfer protein in the Golgi: possible role in the assembly of chylomicrons PMID: 11830580

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  • 亞細胞定位：
    Cytoplasm. Secreted. Lipid droplet.
  • 組織特異性：
    Detected in intestine and liver (at protein level).
  • 數(shù)據(jù)庫鏈接：