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中文名稱:大鼠抗利尿激素/血管加壓素/精氨酸加壓素(ADH/VP/AVP)酶聯(lián)免疫試劑盒
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貨號:CSB-E12684r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠抗利尿激素/血管加壓素/精氨酸加壓素(ADH/VP/AVP)酶聯(lián)免疫試劑盒(CSB-E12684r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AVP含量。AVP 即精氨酸血管加壓素,它在人體的水平衡、血壓調(diào)節(jié)等方面起著重要作用。其研究機制主要圍繞其與受體結(jié)合展開,通過作用于血管、腎臟等部位的受體,調(diào)節(jié)血管收縮、水重吸收等生理過程,目前相關(guān)研究在探索疾病治療方面有一定價值。試劑盒檢測范圍為0.235 ng/ml-15 ng/ml.,靈敏度為0.05 ng/ml.。適用于科研領(lǐng)域探究內(nèi)分泌調(diào)節(jié)機制、腎臟功能研究、高血壓或水代謝異常動物模型的建立與驗證,以及藥物干預(yù)對體液平衡影響的實驗室評估。為神經(jīng)內(nèi)分泌研究、代謝性疾病基礎(chǔ)研究及藥理學(xué)實驗提供靈敏且穩(wěn)定的檢測方案。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:AvpVasopressin-neurophysin 2-copeptin ELISA Kit; AVP-NPII) [Cleaved into: Arg-vasopressin ELISA Kit; Arginine-vasopressin); Neurophysin 2 ELISA Kit; Neurophysin-I); Copeptin] ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.235 ng/ml-15 ng/ml.
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靈敏度:0.05 ng/ml.
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
89
Range %
86-93
1:2
Average %
100
Range %
96-104
1:4
Average %
90
Range %
85-94
1:8
Average %
99
Range %
96-102
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回收率:
The recovery of rat ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
91-98
EDTA plasma (n=4)
98
95-102
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
15
2.348
2.362
2.355
2.182
7.5
1.844
1.871
1.858
1.685
3.75
1.270
1.285
1.278
1.105
1.88
0.686
0.672
0.679
0.506
0.94
0.400
0.388
0.394
0.221
0.47
0.337
0.322
0.330
0.157
0.235
0.248
0.233
0.241
0.068
0
0.174
0.172
0.173
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Comparative analysis of cellular immune responses and cytokine levels in sheep experimentally infected with bluetongue virus serotype 1 and 8. Sánchez-Cordón PJ. et al,Vet Microbiol,2015
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:AVP,又稱抗利尿激素,是一種腦下垂體分泌的激素,能夠調(diào)節(jié)體液平衡和血容量。最近的研究表明,AVP不僅對腎臟和心血管系統(tǒng)有影響,而且對中樞神經(jīng)系統(tǒng)也有影響。研究表明,AVP參與了認知功能和記憶過程的調(diào)節(jié),并可能在抑郁癥等情緒障礙的發(fā)生中發(fā)揮重要作用。此外,AVP與自閉癥譜系障礙的發(fā)生也有一定關(guān)系。
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功能:Neurophysin 2 specifically binds vasopressin.; Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2).
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基因功能參考文獻:
- Anxiolytic Lactobacillus is found to increase in abundance in inverse proportion to increasing arginine-vasopressin (Avp) gene dosage [most abundant in AVP-deficient Brattleboro (KO) rats]. This genotype effect of Lactobacillus abundance is not found when female rats are analyzed independently. Therefore, Avp deletion affects microbiota composition in a sexually differentiated manner. PMID: 28759308
- in the fetal brain whereby vasopressin activates hippocampal interneurons PMID: 29183979
- Studies findings imply that arginine vasopressin has a beneficial role for the treatment of cognitive impairments partly through V1a receptor signaling in cerebral hypoperfusion, which is possibly related to improving synaptic plasticity by promoting N-methyl-d-aspartate receptor subunit 2B and postsynaptic density protein externalization and by enhancing autophagy. PMID: 28934194
- Data (including data from studies in transgenic rats) suggest diabetes is associated with increased AVP in plasma and in hypothalamic magnocellular neurosecretory cells; electrophysiological recordings in supraoptic nucleus suggest that gamma-aminobutyric acid (GABA) functions as excitatory neurotransmitter in AVP neurons from diabetic rats, whereas it evokes inhibitory responses in AVP neurons from control rats. PMID: 29212780
- Vasopressin innervation of the forebrain social behavior neural network undergoes developmental changes and is highly sexually dimorphic. PMID: 28340511
- Study shows that acute amphetamine (AMPH) increased vasopressin (AVP) mRNA expression in the medial amygdala, whereas AMPH-induced sensitisation increased AVP mRNA expression in the bed nucleus of the stria terminalis only in females. PMID: 28235136
- data suggest that GLP-1 combined with vasopressin could be involved in the regulation of sodium balance in the hyperosmolar state as a result of NaCl loading; vasopressin regulates the reabsorption of a significant portion of filtered sodium in the distal segment of the nephron and modulates the natriuretic effect of GLP-1 PMID: 26791475
- Under long term stressed conditions, the release of AVP and OXT from hypothalamus may vary depending on the content of the stressors. Central AVP appears to act oppositely to OXT by mediating CHeS-induced gastric motor maladaptation. Long term central AVP antagonism might be a pharmacological approach for the treatment of stress-related gastric motility disorders. PMID: 27829122
- Aroclor-1254 may interfere with the activation of AVP mRNA transcript levels and protein, causing a central dysfunction of vasopressinergic system. PMID: 28579251
- This study deminstrated that Depressive- and anxiety-like behaviors and stress-related neuronal activation in vasopressin-deficient female Brattleboro rats. PMID: 26939727
- The present study suggests that the Avp gene plays an important role in social development. PMID: 27066536
- The results of this study showed that arginine vasopressin increases in both sexs rats with aneurysmal subarachnoid hemorrhage. PMID: 26923576
- Chronic mild stress increased arginine vasopressin mRNA levels in the paraventricular nucleus. PMID: 26578428
- More AVP was expressed in the paraventricular nucleus after rotatory stimulus in rats susceptible to motion sickness (MS). In the ventricular nucleus, AVP expression were low. Of 9 AVP SNPs, rs105235842 differed significantly with MS susceptibility. PMID: 26651338
- This data provides the connection between CREB3L1, a newly identified transcription factor of AVP expression. PMID: 26503226
- Local cardiac and circulating AVP secretion were enhanced by deteriorating cardiac function. AVP may promote ventricular remodeling. PMID: 25823554
- Regression of Walker 256 carcinosarcoma in vasopressin-deficient Brattleboro rats is accompanied by a changed laminin pattern. PMID: 25854851
- AVP appears to play a role in the regulation of impulsivity exclusively during lactation. PMID: 25117459
- Active coping toward predatory stress decreases DNA methylation in AVP promoter. PMID: 25127982
- Identification of AZIN1, and hence by inference, polyamines as novel regulators of the expression of the AVP gene. PMID: 25961839
- Vasopressin-deficient rats that are deprived of their mothers as newborns do not exhibit stress and anxiety-like behaviors as adults, probably as a consequence of reduced ACTH rise immediately following maternal deprivation. PMID: 25462910
- This study demonstrated that enhanced amygdalar AVP may be related to individual vulnerability to stress-induced reinstatement of heroin- seeking. PMID: 25446223
- Supraoptic nucleus neurons consist of two physiologically distinct populations preferentially expressing oxytocin or vasopressin. PMID: 25451978
- results unveiled a unique and complex form of neurovascular coupling supporting a competitive balance between nitric oxide and activity-dependent dendritic released vasopressin. PMID: 25834057
- The effects of vasopressin on maternal aggression develop in conjunction with impulsivity. PMID: 25001964
- Resistance training did not induce any change in vasopressin levels, and atrial natriuretic peptide levels did not change significantly. However, VP increased after the resting period of 15 days. PMID: 23551170
- UT-A1 localized to the apical membrane in response to vasopressin was phosphorylated at Ser(486) and Ser(499). PMID: 25377918
- eNOS inhibition in spontaneously hypertensive rats induces or enhances an inverse reciprocal interaction between hypothalamus and left ventricle involving the renin-angiotensin system and vasopressin PMID: 24627106
- This study is the first to demonstrate dual vasopressin/apelin adaptation mediated by inflammatory molecules and neuronal Trpv2, during aging. PMID: 24505289
- The endocannabinoid anandamide decreases basal oxytocin and vasopressin secretion from rat neurohypophysis. PMID: 24342802
- We identify CREB3L1 as a regulator of AVP transcription in the rat hypothalamus. PMID: 24623760
- Over-expression of CRH and vasopressin in the magnocellular paraventricular nucleus represents a specific feature of anxiety/post traumatic stress disorder-like state. PMID: 24317347
- Suggest that the P2X7R is expressed in neurohypophysial terminals and has a role in AVP secretion. PMID: 24037803
- The positive role of arginine vasopressin (AVP) in the regulation of adrenocorticotropic hormone secretion from the pituitary in animals exposed to heat is confirmed. AVP from the general circulation influences pituitary vasopressin 1b receptor. PMID: 23580725
- Arginine vasopressin-containing neurons of the magnocellular hypothalamic nuclei serve as important sources for hippocampal innervation. PMID: 23085097
- The Notch1-Hes1 signalling axis suppresses neuroendocrine differentiation and maintains tubular/acinar features in adenocarcinoma and non-neoplastic epithelium in the biliary tree. PMID: 23523151
- Interactions between corticosterone and catecholaminergic projections to the hypothalamus therefore make significant contributions to the regulation of Crh and Avp expression. PMID: 22831701
- Leukotrienes may modulate arginine vasopressin secretion. PMID: 22960410
- GLP-1 directly stimulated the promoter activities and mRNA levels of both CRF and AVP in hypothalamic 4B cells PMID: 22801106
- These results that AVP excites warm-sensitive neurons and inhibits cold-sensitive and temperature-insensitive neurons PMID: 22677202
- No difference is found in either quality or quantity of oxytocin- and vasopressin-immunoreactive neurons between individual rats of different social and reproductive ranks. PMID: 22266748
- These data demonstrate direct relationships between enhanced AVP neuronal activation and a potentiated vasopressin system, and this latter one with high conditioned anxiety in MS male rats PMID: 22522466
- Chronic psychological stress disrupted fasted motility in the antrum and colon via central urocortin 3/arginine vasopression pathways. PMID: 22651925
- Vasopressin was more important in the regulation of the adrenocorticotropin secretion in the perinatal vasopressin-deficient Brattleboro rats than in adulthood, where its role depended on the nature of the stressor encountered. PMID: 22527858
- In the mammalian peripartum period, the activity of both the brain oxytocin and vasopressin system is elevated as part of the physiological adaptations occurring in the mother--REVIEW PMID: 22100184
- We review this and other work demonstrating that both vasopressin and oxytocin modulate conspecific social recognition at the level of the olfactory system--review PMID: 21920364
- initial findings suggest that oxytocin and vasopressin systems may mediate early social environment-induced alterations in social behavior--REVIEW PMID: 22197269
- AVP can act within multiple regions social behavior network to regulate the expression of individual social behaviors--REVIEW PMID: 22079778
- During protracted withdrawal from cocaine, enhanced AVP gene expression is associated with persistent elevations of basal hypothalamus pituitary axis activity. PMID: 21677651
- AVP secretion of the pituitary may be involved in the sex differences in susceptibility to motion sickness. PMID: 21171363
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亞細胞定位:Secreted.
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蛋白家族:Vasopressin/oxytocin family
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