Rat beta-crosslaps (bCTx) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠β膠原交聯(lián)(bCTx)酶聯(lián)免疫試劑盒（CSB-EQ027521RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的bCTx含量。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，在骨質(zhì)疏松機(jī)制研究、骨病模型評價(jià)及骨代謝相關(guān)藥物研發(fā)中具有重要科研價(jià)值；廣泛應(yīng)用于骨代謝動態(tài)監(jiān)測、抗骨吸收藥物療效評估、骨折愈合研究及骨腫瘤模型構(gòu)建等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

bCTx
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	93-99
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	84-90

回收率：

The recovery of rat bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	97-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.