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  4. Rat cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA kit

Rat cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA kit

  • 中文名稱：
    大鼠細胞色素P450,家族7亞科A多肽1(CYP7A1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL006460RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠細胞色素P450,家族7亞科A多肽1(CYP7A1)酶聯免疫試劑盒（CSB-EL006460RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CYP7A1含量。CYP7A1 是膽固醇代謝中的關鍵靶點。其背景在于它是肝臟膽固醇轉化為膽汁酸的限速酶。研究機制方面，主要聚焦于其在調節(jié)膽固醇穩(wěn)態(tài)、脂質代謝中的作用，通過調控它的表達和活性，為治療高膽固醇血癥、脂肪肝等疾病提供潛在策略。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，本試劑盒可精準量化其表達水平，為探索膽固醇代謝通路、肝臟生理機制及藥物代謝相關研究提供可靠工具。適用于體外實驗中對CYP7A1蛋白表達變化的動態(tài)監(jiān)測，如膽汁酸合成研究、代謝性疾病模型分析或藥物干預實驗中的生物標志物檢測，滿足基礎科研中對靶標分子定量分析的需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Cyp7a1 ELISA kit; Cyp7Cytochrome P450 7A1 ELISA kit; 24-hydroxycholesterol 7-alpha-hydroxylase ELISA kit; EC 1.14.14.26 ELISA kit; CYPVII ELISA kit; Cholesterol 7-alpha-hydroxylase ELISA kit; Cholesterol 7-alpha-monooxygenase ELISA kit; EC 1.14.14.23 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.4 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP7A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-92  
    1:2 Average % 96  
    Range % 92-99  
    1:4 Average % 98  
    Range % 93-103  
    1:8 Average % 95  
    Range % 90-98  
  • 回收率：
    The recovery of rat CYP7A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 96 92-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    6000 2.467 2.414 2.441 2.349  
    3000 2.011 1.999 2.005 1.913  
    1500 1.594 1.584 1.589 1.497  
    750 1.085 1.067 1.076 0.984  
    375 0.683 0.672 0.678 0.586  
    187.5 0.401 0.387 0.394 0.302  
    93.75 0.235 0.221 0.228 0.136  
    0 0.093 0.091 0.092 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.
  • 基因功能參考文獻：
    1. lnc-HC negatively regulates cholesterol metabolism within hepatocytes through physical interaction with hnRNPA2B1. By further binding to the target messenger RNA of Cyp7a1 or Abca1, the lnc-HC-hnRNPA2B1 complex decreases expressions of the two genes that are implicated in cellular cholesterol excretion. PMID: 26663205
    2. Administration of glucocorticoids (GCs) enhanced liver bile acid uptake by increasing the expression of the major hepatocyte basolateral bile transporter (Ntcp) in rats. The reduced expression of a bile acid synthesis rate-controlling enzyme, Cyp7a1, suggests that treatment with GCs suppressed hepatic bile acid synthesis. PMID: 27133208
    3. Dietary fats and glucomannan plus spirulina added to pork significantly alters Cyp7a1 expression in Zucker rats. PMID: 26475369
    4. Ginsenosides promoted liver cholesterol metabolism due to upregulation of CYP7A1. PMID: 23108811
    5. Portal blood FGF15/19 levels remain unchanged while the corresponding ileal mRNA and liver CYP7A1 levels change significantly in cholesterol fed rats. PMID: 23852734
    6. Capsaicinoids consumption down-regulated the mRNA levels of HMG-CoA reductase and CYP7A1 in the cholesterol-free diet group but up-regulated the CYP7A1 level in the cholesterol-enriched diet group. PMID: 23617777
    7. insulin-dependent suppression results in impairment of the hepatic cholesterol/bile acid metabolisms PMID: 21817852
    8. Maternal protein restriction elevates cholesterol in adult rat offspring due to repressive changes in histone modifications at the cholesterol 7alpha-hydroxylase promoter PMID: 21372147
    9. Cytochrome P450 7A1 cholesterol 7alpha-hydroxylation: individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction. PMID: 21147774
    10. essential for heart and skeletal muscle function PMID: 20215401
    11. The mRNA and protein expressions of SHP and CYP7A1 were increased in livers of pregnant rats with intrahepatic cholestasis. PMID: 18578998
    12. Cyp7a1 activity is modified after chronic ingestion of depleted uranium in the rat. PMID: 20362056
    13. The increased recruitment of LXRalpha, a Cyp7a1 stimulatory pathway, and decreased expression of FGF15 and phosphorylated Erk1/2, a Cyp7a1 repressive pathway, combined to increase Cyp7a1 expression during lactation. PMID: 19957370
    14. LXR alpha is the dominant regulator of CYP7A1 transcription PMID: 12054605
    15. transcription suppressed by alpha1-antitrypsin peptide via interaction with AFP PMID: 12223476
    16. Data reveal a fundamental difference in the regulation of CYP7A1 in rodent and human hepatocytes. PMID: 12554795
    17. ARP-1/COUP-TFII acts as a transcription activator of CYP7A1. PMID: 12777384
    18. results suggest that the mechanisms of regulation of these bile acid metabolizing enzymes are significantly different during liver regeneration PMID: 16081591
    19. These results suggest that the cyp7A1 gene plays an important role in development of hypercholesterolemia in Prague hereditary hypercholesterolemic rats. PMID: 18226361
    20. Increased Cyp7a1 expression inlactating rats provides a mechanism for the increase in the bile acid pool. PMID: 18292185
    21. The effect of osthole on protein expression of PPARalpha/gamma and related target molecules such as CYP7A and DGAT protein expression in the liver of hyperlipidemic fatty liver rats. PMID: 18696335
    22. A relationship between the decreased expression of hepatic CYP7A1 mRNA and protein and dilated ER may contribute to hypercholesterolemia in chronic hypothyroidism. PMID: 19788618

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 組織特異性：
    Detected in liver (at protein level). Liver.
  • 數據庫鏈接：