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  4. Rat diamine oxidase(DAO) ELISA Kit

Rat diamine oxidase(DAO) ELISA Kit

  • 中文名稱：
    大鼠二胺氧化酶(DAO)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12634r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠二胺氧化酶(DAO)酶聯(lián)免疫試劑盒（CSB-E12634r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的DAO含量。DAO即去中心化自治組織，背景是隨著區(qū)塊鏈技術(shù)發(fā)展應(yīng)運而生，旨在以智能合約實現(xiàn)組織的自治管理。其研究機制圍繞如何通過代碼和規(guī)則確保組織運作，減少人為干預(yù)，實現(xiàn)公平、透明決策，在金融、社區(qū)治理等領(lǐng)域有廣泛應(yīng)用前景。試劑盒檢測范圍為0.78 mIU/mL-50 mIU/mL，適用于腸道疾病模型研究、腸屏障功能評估、炎癥機制探索等科研領(lǐng)域，例如通過檢測實驗動物血清或組織樣本中DAO水平，研究腸道損傷修復(fù)、藥物干預(yù)效果或病理生理過程。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC 1.4.3.3 ELISA Kit
  • 縮寫：
    DAO
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.78 mIU/mL-50 mIU/mL
  • 靈敏度：
    0.195 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %85
    Range %80-91
    1:200Average %93
    Range %89-99
    1:400Average %87
    Range %82-95
    1:800Average %101
    Range %98-104
  • 回收率：
    The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-92
    EDTA plasma (n=4)9085-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml. OD1OD2AverageCorrected
    502.634 2.672 2.653 2.459
    252.267 2.298 2.283 2.089
    12.51.706 1.765 1.736 1.542
    6.251.114 1.156 1.135 0.941
    3.120.623 0.672 0.648 0.454
    1.560.492 0.480 0.486 0.292
    0.780.307 0.322 0.315 0.121
    00.193 0.195 0.194 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
  • 基因功能參考文獻：
    1. Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
    2. Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
    3. The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
    4. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
    5. results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
    6. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
    7. These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858

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  • 亞細胞定位：
    Peroxisome.
  • 蛋白家族：
    DAMOX/DASOX family
  • 數(shù)據(jù)庫鏈接：