Rat enkephalin,ENK ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠腦啡肽(ENK)酶聯(lián)免疫試劑盒（CSB-E13325r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ENK含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于神經(jīng)科學、藥理學等領域的基礎研究，例如探究疼痛機制模型、應激反應調(diào)控或藥物干預對ENK表達的影響，為神經(jīng)系統(tǒng)功能研究提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ENK
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	103-111
1:2	Average %	95
	Range %	92-98
1:4	Average %	90
	Range %	86-95
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of rat ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	91	85-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.