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  4. Rat fatty acid synthase (FAS) ELISA kit

Rat fatty acid synthase (FAS) ELISA kit

  • 中文名稱：
    大鼠脂肪酸合成酶(FAS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16440r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠脂肪酸合成酶(FAS)酶聯(lián)免疫試劑盒（CSB-E16440r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FASN含量。FASN 全稱脂肪酸合酶，它在脂肪酸合成中起關鍵作用。在腫瘤細胞中，F(xiàn)ASN 常高表達以滿足其快速增殖的代謝需求。研究機制方面，主要通過抑制 FASN 活性來阻斷腫瘤細胞脂肪酸合成，干擾其細胞膜形成、能量代謝等過程，成為潛在抗癌靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于探索代謝性疾病發(fā)生機制、評估營養(yǎng)干預對脂代謝的影響，或作為藥物開發(fā)中靶向FAS通路的生物標志物分析工具；支持科研人員在肝臟代謝研究、脂肪組織功能評估或能量代謝相關動物模型實驗中快速獲取穩(wěn)定可靠的FAS濃度數(shù)據(jù)，為深入揭示脂質(zhì)合成調(diào)控網(wǎng)絡提供實驗支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FasnFatty acid synthase ELISA kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA kit; EC 3.1.2.14)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-94
    1:2Average %92
    Range %88-99
    1:4Average %88
    Range %84-92
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10096-108
    EDTA plasma (n=4)9790-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    1002.572 2.427 2.500 2.371
    501.837 1.902 1.870 1.741
    251.353 1.308 1.331 1.202
    12.50.775 0.819 0.797 0.668
    6.250.512 0.516 0.514 0.385
    3.120.305 0.294 0.300 0.171
    1.560.245 0.251 0.248 0.119
    00.131 0.127 0.129
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
  • 基因功能參考文獻：
    1. Calorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. PMID: 28810876
    2. The involvement of SREBP-1c and ChREBP in FASN promoter histone modification.Histone acetylation affects FASN transcription by influencing ChREBP-binding carbohydrate-responsive elements. PMID: 28027934
    3. This study showed that an associated inhibition of peroxisomal proliferator activated receptor gamma coactivator 1alpha (PGC1alpha), increased fatty acid synthase (FASN) expression in the liver, and reduced adipocyte triglyceride lipase (ATGL) expression. PMID: 26287659
    4. Data suggest expression of Fas in liver can be regulated by dietary components; dietary fructose up-regulates expression of Fas; supplementation with citrulline prevents up-regulation of Fas, insulin resistance, and nonalcoholic fatty liver disease. PMID: 26246323
    5. Results show that genes with the highest expression in the mammary gland, liver and adipose tissue were Elovl5, Fads2, and Fasn, respectively, in a low-lipid diet than in adequate-lipid diet. PMID: 25046614
    6. Epididymal adipose tissue expression of the fatty acid synthase (FASN) gene and NADH dehydrogenase (ubiquinone) 1beta-subcomplex 6 (NDUFB6) were significantly reduced in the high fat diet group PMID: 20729114
    7. suppression of promoter by polyunsaturated fatty acids PMID: 11971939
    8. Transcription factors act on the promoter of the rat fatty acid synthase gene [review] PMID: 12440974
    9. hepatic gene expression of acetyl-coenzyme A carboxylase and fatty acid synthase was increased 2-fold in late low protein and low protein offspring PMID: 12634446
    10. glycerol-3-phosphate dehydrogenase, fatty acid synthase, and acetyl-coa carboxylase mRNA levels are upregulated by glucose, which requires glutamine, and glucosamine PMID: 12759350
    11. Downregulated by rosiglitazone. PMID: 15887043
    12. Prolactin represses expression of transfected rat FAS in mouse adipocytes through STAT5A binding to the -908 to -893 site. PMID: 15983196
    13. Insulin acutely reduces hepatic FAS activity by inducing phosphorylation of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and its interaction with FAS. PMID: 16054098
    14. These results showed that increases in SREBP-1c were neither necessary nor sufficient for Glucokinase induction in hepatocytes, while at the same time they underscored the role of SREBP-1c as a key regulator of Fatty acid synthase. PMID: 16834571
    15. polyunsaturated fatty acids decrease in vivo binding of NF-Y and SREBP-1c to the proximal promoter of the hepatic Fasn gene PMID: 17313375
    16. Insulin, glucose and AICAR (an activator of AMP-activated protein kinase) affected changes in hypothalamic FAS mRNA, which may be regulated via the SREBP1c dependent or independent pathway. PMID: 17709201
    17. Normal flux through Fasn is not required for glucose-stimulated insulin secretion. PMID: 17823126
    18. Findings establish that sequence elements previously assigned to a central structural core region of the type I fatty acid synthases and some modular polyketide synthase counterparts play an essential catalytic role as part of the dehydratase domain. PMID: 18096506
    19. Dietary soy protein, by decreasing circulating insulin levels and colon FASN expression, attenuates insulin-induced DNA damage and FASN-mediated anti-apoptosis during carcinogenesis. PMID: 18239060
    20. There were reduced nuclear levels of the lipogenic transcription factor sterol regulatory element binding protein-1 in MCD-fed mice, but no consistent reduction in fatty acid synthesis genes (acetyl-CoA carboxylase and fatty acid synthase). PMID: 18280001
    21. Results describe the inhibition of fatty acid synthase by flavonoids extracted from Pangdahai (Sterculia). PMID: 18380011

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  • 亞細胞定位：
    Cytoplasm. Melanosome.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:50671

    STRING: 10116.ENSRNOP00000064445

    UniGene: Rn.9486