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  4. Rat gastric inhibitory polypeptide (Gip) ELISA kit

Rat gastric inhibitory polypeptide (Gip) ELISA kit

  • 中文名稱：
    大鼠抑胃肽(GIP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17969r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠抑胃肽(GIP)酶聯(lián)免疫試劑盒（CSB-E17969r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Gip含量。GIP（葡萄糖依賴性促胰島素多肽）是一種由腸道K細(xì)胞分泌的腸促胰島素，主要分布在十二指腸和近端空腸，對(duì)食物攝取后胰島素的分泌起到重要調(diào)節(jié)作用。GIP通過與其受體結(jié)合，激活信號(hào)通路，刺激胰島β細(xì)胞分泌胰島素，同時(shí)在胰島α細(xì)胞中促進(jìn)胰高血糖素的分泌，從而在血糖調(diào)節(jié)中發(fā)揮智能調(diào)節(jié)作用。GIP的研究對(duì)于理解糖尿病和肥胖等代謝疾病的發(fā)病機(jī)制具有重要意義。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，可滿足科研場(chǎng)景中對(duì)GIP動(dòng)態(tài)變化的檢測(cè)需求，例如研究腸道激素分泌調(diào)控機(jī)制、評(píng)估營(yíng)養(yǎng)干預(yù)或藥物處理對(duì)大鼠代謝狀態(tài)的影響等。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GipGastric inhibitory polypeptide ELISA kit; GIP ELISA kit; Glucose-dependent insulinotropic polypeptide ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Gip in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 87-96  
    1:2 Average % 99  
    Range % 93-105  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of rat Gip spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-102  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.812 2.732 2.772 2.599  
    200 1.965 1.901 1.933 1.760  
    100 1.215 1.247 1.231 1.058  
    50 0.713 0.753 0.733 0.560  
    25 0.516 0.531 0.524 0.351  
    12.5 0.433 0.409 0.421 0.248  
    6.25 0.257 0.241 0.249 0.076  
    0 0.176 0.169 0.173 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • 基因功能參考文獻(xiàn)：
    1. Sitagliptin attenuated resistin expression via the GIP-dependent pathway, which inhibited sympathetic innervation through a signalling pathway involving phosphatidylinositol 3-kinase (PI3K) and Akt protein. PMID: 26811539
    2. provide evidence that gastric inhibitory polypeptide regulates skeletal muscle glucose transport; however, the exact signaling mechanism(s) remain unknown PMID: 26041107
    3. glucose-dependent insulinotropic polypeptide and Glucose-dependent insulinotropic polypeptide receptors expression in the rat major salivary glands, as well as its variation in the rat SMG during the growth period PMID: 24360021
    4. The role of GIP as a critical link between catch-up growth and the development of metabolic disturbances. PMID: 25032669
    5. In a HFD model, DPP4-rats exhibited reduced adipose tissue inflammation and improved insulin resistance, which may be mediated in part by GIP induction of adiponectin. PMID: 23408696
    6. Changes in the secretion and action of GIP play an important role in metabolic adaptations during pregnancy and especially lactation. PMID: 24236022
    7. Dietary fructose elicits GLP-1 secretion without simultaneous release of glucagonotropic GIP. PMID: 24525020
    8. Data suggest that secretion of GIP and GLP1 (glucagon-like peptide-1) by enterocytes (in rats with diabetes type 2 and in normal rats) can be up-regulated by dietary factors, here, corn zein hydrolysate as a hypoglycemic dietary supplement. PMID: 23798598
    9. Postprandial plasma GIP response is blunted in rats fed chlorogenic acid (a component of coffee), with a lower peak concentration and decreased AUC up to 180 min. PMID: 21977912
    10. Plasma glucose and insulin concentrations were not affected by oral fat, but fat-induced Gip secretion was particularly (p < 0.05) increased in insulinoma-bearing rats. PMID: 21851286
    11. truncated forms of GIP exhibit potent anti-diabetic actions, without pro-obesity effects, and that the C-terminus contributes to the lipogenic actions of GIP PMID: 20231880
    12. Both GIP and GLP-1 outputs responded dose dependently to increasing amounts of dietary carbohydrate but not protein. GIP-secreting cells were more sensitive than GLP-1-secreting cells to changes in intestinal carbohydrate content. PMID: 20522638
    13. GIP is expressed in and secreted from pancreatic islets and promotes islet glucose competence and also could support islet development and/or survival. PMID: 20138041
    14. GIP, GLP-1, insulin and gastrin have roles in ghrelin release in the isolated rat stomach PMID: 15093702
    15. Persistent activation (8 h) of the GIP signalling pathway decreased the GLP (glucagon-like peptide)-1 dependent insulin secretion accompanied by an upregulation of G-protein alphai2 protein level to about 126% PMID: 15376236
    16. Hippocampal expression of the gene-encoding varied strongly in parallel with cell-proliferation rates in the adult rat dentate gyrus. PMID: 15716418
    17. These results indicate that cell-specific expression of GIPR is associated with the binding of the transcription factors Sp1 and Sp3 to the GIPR promoter. PMID: 16403775
    18. Prohormone convertase 1/3 is essential for processing of the glucose-dependent insulinotropic polypeptide precursor PMID: 16476726
    19. protein kinase B, LKB1, and AMP-activated protein kinase have roles in activation of lipoprotein lipase by glucose-dependent insulinotropic polypeptide in adipocytes PMID: 17244606
    20. Administration of resistin to adipocytes mimicked the effects of GIP on the PKB/LKB1/AMPK/LPL pathway: increasing phosphorylation of PKB, reducing levels of phosphorylated LKB1 and AMPK, and increasing LPL activity. PMID: 17890220
    21. data indicate a modest effect of GIP on glucagon secretion from isolated rat islets, which was not observed in vivo PMID: 18163884
    22. GATA-4 may function to augment or enhance GIP expression rather than act as an initiator of GIP transcription. PMID: 18343025
    23. feeding rats with dietary resistant starch reduced the GIP mRNA levels along the entire length of the jejunoileum in both Wistar and type 2 diabetic GK rats PMID: 18685191
    24. Nutrient-driven GIP secretion into intestinal lymph is different between diabetic Goto-Kakizaki rats and Wistar rats. PMID: 19056762
    25. oral glucose but not artificial sweetners increased plasma GIP levels approximately 4-fold and GLP-1 levels approximately 2.5-fold postadministration PMID: 19106249
    26. Analyses with GIPR-deficient mice suggest a role of GIP/GIPR signal transduction in promoting spontaneous recovery after nerve crush; injury of GIPR-deficient mouse sciatic nerve revealed impaired axonal regeneration. PMID: 19170165
    27. Data show that glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 stimulated Akt/protein kinase B in the INS-1 beta-cell line and isolated mouse islets via a mechanism that did not require phosphoinositide 3-kinase. PMID: 19233842
    28. GIP secretion into lymph is stimulated dose dependently by dietary lipids. PMID: 19520739

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Glucagon family
  • 組織特異性：
    Highly expressed in the duodenum and jejunum.
  • 數(shù)據(jù)庫(kù)鏈接：