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  4. Rat glial cell line-derived neurotrophic factor,GDNF ELISA KIT

Rat glial cell line-derived neurotrophic factor,GDNF ELISA KIT

  • 中文名稱：
    大鼠膠質(zhì)細(xì)胞系來(lái)源的神經(jīng)營(yíng)養(yǎng)因子(GDNF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04566r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠膠質(zhì)細(xì)胞系來(lái)源的神經(jīng)營(yíng)養(yǎng)因子(GDNF)酶聯(lián)免疫試劑盒（CSB-E04566r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的GDNF含量。GDNF（膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子）是一種神經(jīng)營(yíng)養(yǎng)因子，屬于TGF-β超家族，對(duì)中樞和周圍神經(jīng)系統(tǒng)中多種神經(jīng)元亞群的存活和功能維持至關(guān)重要。其研究機(jī)制涉及促進(jìn)神經(jīng)元存活、突起生長(zhǎng)和神經(jīng)肌肉連接，在神經(jīng)損傷修復(fù)和神經(jīng)退行性疾病治療中具有潛在應(yīng)用價(jià)值。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，科研人員可通過(guò)定量分析體外培養(yǎng)神經(jīng)細(xì)胞分泌的GDNF動(dòng)態(tài)變化，或評(píng)估帕金森病模型動(dòng)物外周血中GDNF的表達(dá)調(diào)控，為神經(jīng)保護(hù)機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)及神經(jīng)再生相關(guān)基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    GdnfGlial cell line-derived neurotrophic factor ELISA Kit; Astrocyte-derived trophic factor ELISA Kit; ATF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess. 
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess. 
     
  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat GDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

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  • 回收率：

    The recovery of rat GDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. May also modulate local neuronal effects in distal regions of the motor neuron.
  • 基因功能參考文獻(xiàn)：
    1. the inverse relationship between GFRalpha1 and C-Ret, as knocking down C-Ret led to increases in GFRalpha1 expression. PMID: 29018141
    2. considerable structure-specific differences in GDNF expression between highly aggressive and nonaggressive rats were shown PMID: 27519646
    3. findings indicate that EC-PAR acts as a neurotrophic enhancer by upregulating GDNF levels. This effect protects the remaining neurons following oxidative stress-induced brain damage, such as that seen with Parkinson disease PMID: 28130107
    4. In addition, amyotrophic lateral sclerosis (ALS) rats receiving GDNF exhibited slower weight gain, reduced activity levels and decreased working memory. Collectively, these results confirm that caution should be applied when applying growth factors such as GDNF systemically to multiple tissues. PMID: 28276446
    5. hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression PMID: 27230884
    6. This study demonstrated that GDNF regulated dopamine transporter activity without affecting tyrosine hydroxylase expression in the rat mesostriatal system. PMID: 26777664
    7. Reverse transcription-quantitative PCR and western blot analysis demonstrated that there was an increase in the mRNA and protein expression of NGF and GDNF, both of which are markers of SC neurotrophic secretion PMID: 26709397
    8. In neuropathic pain treated with pulsed radiofrequency, pain reduction was associated with GDNF up-regulation. PMID: 26815249
    9. Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model. PMID: 26466815
    10. GDNF increases the intracellular phosphorylation level of beta-catenin through N-cadherin/Src signaling, which subsequently stimulates the nuclear translocation of beta-catenin PMID: 25423132
    11. GDNF administration into the Locus Coeruleus provides long-lasting pain relief by recruiting descending noradrenergic inhibition. PMID: 25572945
    12. SHH but not GDNF controls the phenotypic fate and therapeutic efficacy of grafted neural precursor cells in a model of nigrostriatal neurodegeneration. PMID: 26340267
    13. Expression levels of the Gdnf gene may modify the severity of HSCR in rats carrying Ednrb(sl) mutations. PMID: 26318480
    14. Results show that in the inflamed intestine, smooth muscle proliferation supports the enteric nervous system , and thus its own re-innervation, by expression of GDNF PMID: 25655216
    15. The effects of glial cell-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) on the course of bone marrow mesenchymal stem cell differentiation. PMID: 25301495
    16. Electroacupuncture with high-frequency and long-term stimuli at acupoint ST-36 can induce regeneration of lost enteric neurons in diabetic rats by GDNF/PI3K/AKT signaling. PMID: 25972459
    17. GDNF plays an important role in the barrier-protective mechanism of activated enteric glial cells under ischemia reperfusion stimulation PMID: 24878766
    18. the PTX-sensitive alpha-subunit of G proteins, Go1 and Gi3, plays an important role in a monoamine-independent production of GDNF evoked by amitriptyline PMID: 25869129
    19. These results suggest that D2R activation induces a Gbetagamma- and extracellular signal-regulated kinase 1/2-dependent increase in the level of Zif268, which functions to directly up-regulate the expression of GDNF. PMID: 23373701
    20. histone H3K9 hyperacetylation in promoter II of the gdnf gene might be one of the reasons for its abnormal high transcription in glioma cells. PMID: 24619502
    21. Glial cell line-derived neurotrophic factor is a secreted growth factor, essential for the development, differentiation, proliferation and function of midbrain dopaminergic neurons. PMID: 23298382
    22. Differences of GDNF expression are linked to the differential timing of RLN axon regeneration and individual muscle reinnervation. PMID: 25220603
    23. The hyperacetylation of H3K9 at Egr-1 binding sites in promoter region II of the gdnf gene can up-regulate the binding of Egr-1 to increase gdnf gene transcription in glioma cells. PMID: 25201174
    24. ProGDNF is a biologically active protein with specific effects on the cells close to its secreting site and in the glia-neuronal interaction. PMID: 23934644
    25. The present study indicates that genetically modified BMSCs co-expressing GDNF and NT-3 are able to differentiate into enteric neuronal cells and express enteric nerve markers when induced by FGCM. PMID: 22282251
    26. Co-transplantation of GDNF-overexpressing neural stem cells and fetal dopaminergic neurons mitigates motor symptoms in a rat model of Parkinson's disease. PMID: 24312503
    27. There was no direct effect of H2O2 or l-DOPA on striatal GDNF levels. PMID: 24632468
    28. Data suggest that a dietary factor (here, docosahexaenoic acid-DHA) can up-regulate Gdnf activity in hippocampal neurons of the natural aged female rat; DHA improves oxidative parameters and activates the Gdnf/Gfra1-MAPK pathway. PMID: 23767459
    29. In conclusion, the CEXA and Pyr are brain structures sensitive to alcohol-induced imbalance in neurotrophic factors expression; and BALs are not a mirror of BrALs. PMID: 23588198
    30. data collectively indicate that GDNF is capable of inducing anti-oxidant activities of intra-cellular GPX-1 and that growth-promoting potential of GDNF and anti-oxidant properties of GPX-1 can, in concert, maximize survival of dopaminergic neurons. PMID: 23472740
    31. a novel role for the niche-derived factor GDNF PMID: 23613711
    32. The secretion of GDNF, but not BDNF, by the transplanted neural stem cells might contribute to the relief of neuropathic pain. PMID: 23694790
    33. BDNF/GDNF secreted by olfactory bulb cells protects neural stem cells from salsolinol induced apoptotic changes. PMID: 23261589
    34. Cell migration and differentiation of neural stem cells are associated with expression of GDNF but do not prevent cell death. PMID: 22985671
    35. GDNF expression in astrocytes is induced by adenosine A2a receptor through the signal transducer and activator of transcription (Stat)3 pathway. PMID: 23032401
    36. An alternative NF-kappaB signaling pathway is activated by GDNF and is translocated into the nuclei of neuroprotective dopaminergic neurons. PMID: 22230667
    37. Our results demonstrate that activated microglia exert a neuroprotective role through balancing the expressions of GDNF and TNF-alpha after subacute cerebral ischemia. PMID: 23151666
    38. Ultrasound-induced dissolution of lipid-coated GDNF microbubbles treatment reduces infarction volume and improves neurological outcomes in neonatal rats. PMID: 22579834
    39. astrocytic nAChRs play a role in the neuroprotection against different cytotoxins after predominant upregulation of GDNF expression PMID: 22807215
    40. The phosphatidylinositol (PI)3-kinase/Akt pathway plays a positive role in fibroblast growth factor (FGF)-2-stimulated GDNF release independently of p44/p42 MAP kinase or SAPK/JNK in C6 glioma cells. PMID: 22575563
    41. GDNF is an important player in 17beta-oestradiol-mediated dopaminergic neuroprotection. PMID: 22672424
    42. Wide band frequency noise can reduce the expression of GDNF in temporal lobe of Alzheimer Disease model rats. PMID: 21174801
    43. These data support the suggestion that GDNF induces nociceptor sensitization and mechanical hyperalgesia PMID: 22704965
    44. lipopolysaccharide-induced nigral inflammation led to marked elevation of IL-1beta but insignificant TNF-alpha tissue content and to prominent expression of GDNF in astrocytes but not microglia; results suggest inflammation is not only involved in neuronal loss but could promote neuronal survival through increased release of GDNF following upregulation of IL-1beta PMID: 22281445
    45. The potential effects of GDNF and its receptors in experimental diabetic retinopathy indicate that GDNF may exert different effects on cultured Muller cells under high glucose conditions. PMID: 22266674
    46. GDNF protects enteric glia from apoptosis. PMID: 22251670
    47. By increasing GDNF expression in astroglia and by reducing release of pro-inflammatory factors from activated microglia, amantadine protects dopamine neurons in two different models of Parkinson's disease. PMID: 21586298
    48. Glial cell line-derived neurotrophic factor (GDNF)-pretreatment of neural stem cells enhances grafted cell survival in a rat model of Parkinson's disease. PMID: 21699926
    49. Data support hypothesis that cyclical changes in GDNF expression by Sertoli cells (primary source of GDNF here) are responsible for stage-specific replication and differentiation of adult stem cells and spermatogonia from seminiferous epithelium. PMID: 21653894
    50. These results robustly demonstrate that GDNF is essential for the electroconvulsive shock neuroprotective effect observed in 6-OHDA-injected animals. PMID: 21871541

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family, GDNF subfamily
  • 組織特異性：
    Expressed in both the central nervous system (CNS) and in non-CNS tissues, including the kidney, lung, bone, heart, liver, spleen, sciatic nerve and blood. Expressed in brain (at protein level). Localizes at the proximal ligature of the hypoglossal nerve.
  • 數(shù)據(jù)庫(kù)鏈接：