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  4. Rat heparin-binding EGF-like growth factor (HBEGF) ELISA kit

Rat heparin-binding EGF-like growth factor (HBEGF) ELISA kit

  • 中文名稱：
    大鼠肝素結(jié)合EGF樣生長(zhǎng)因子(HBEGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL010154RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠肝素結(jié)合EGF樣生長(zhǎng)因子(HBEGF)酶聯(lián)免疫試劑盒（CSB-EL010154RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HBEGF含量。HBEGF是一種重要的靶點(diǎn)。它是表皮生長(zhǎng)因子家族成員，在細(xì)胞生長(zhǎng)、增殖和遷移等過(guò)程中發(fā)揮作用。研究聚焦于其在腫瘤發(fā)生、發(fā)展中的機(jī)制，比如促進(jìn)腫瘤細(xì)胞生長(zhǎng)、侵襲和轉(zhuǎn)移等，有望為癌癥等疾病治療提供新方向。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于科研領(lǐng)域研究HBEGF在肝損傷、心血管疾病或腫瘤模型中的表達(dá)調(diào)控機(jī)制，例如通過(guò)檢測(cè)肝纖維化模型中組織勻漿HBEGF水平變化，或分析藥物干預(yù)后血漿因子動(dòng)態(tài)變化，為探索HBEGF相關(guān)信號(hào)通路及疾病機(jī)理提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Hbegf; Dtr; Hegfl; Proheparin-binding EGF-like growth factor
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HBEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 91-108
    1:2 Average % 100
    Range % 91-105
    1:4 Average % 94
    Range % 87-99
    1:8 Average % 98
    Range % 90-103
  • 回收率：
    The recovery of rat HBEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 81-95
    EDTA plasma (n=4) 102 95-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    400 2.727 2.586 2.657 2.537
    200 2.229 2.179 2.204 2.084
    100 1.452 1.428 1.440 1.320
    50 0.739 0.727 0.733 0.613
    25 0.423 0.411 0.417 0.297
    12.5 0.268 0.261 0.265 0.145
    6.25 0.182 0.175 0.179 0.059
    0 0.118 0.122 0.120 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • 基因功能參考文獻(xiàn)：
    1. Heterotrimeric G proteins directly regulate membrane-localized MMP14/MT1-MMP, resulting in HB-EGF release and EGFR transactivation. PMID: 25759388
    2. HB-EGF promotes hepatic stellate cell proliferation via activation of the EGFR and ErbB4 receptors PMID: 24303948
    3. FAK phosphorylation and FAK-mediated signal transduction play essential roles in HB-EGF-mediated intestinal epithelial cell migration and adhesion. PMID: 24703506
    4. Intraluminal administration of HB-EGF reduces the severity of intestinal congestion/repurfusion(C/R) injury in rats. PMID: 23534509
    5. HB-EGF induces cardiomyocyte hypertrophy through an EGFR-ERK5-MEF2A-COX-2 pathway. PMID: 21244855
    6. There were no significant changes in HB-EGF mRNA or protein expression in either the plantaris or the soleus in response to functional overload. PMID: 20696782
    7. HB-EGF contributes to vascular remodeling. PMID: 11882602
    8. HB-EGF expressed in proximity to developing mesencephalic dopaminergic neurons promotes their survival in vitro. HB-EGF may be an important molecule for developing dopaminergic neurons of the ventral midbrain. PMID: 11956950
    9. data indicate HB-EGF gene expression can be regulated by Cdx2 in intestinal epithelial (IEC-6) cells PMID: 12223343
    10. Data suggest that the interaction between HB-EGF and EGFR transactivation is closely related to the proliferation of cardiac fibroblasts and cardiac remodeling after myocardial infarction. PMID: 12237129
    11. HB-EGF mRNA expression and protein production in intestine undergoing ischemia/reperfusion injury PMID: 12746188
    12. Chelerythrine stimulated production of reactive oxygen species, and antioxidants prevented chelerythrine-induced HB-EGF shedding, suggesting that the production of intracellular peroxides is necessary for this event PMID: 15490481
    13. analysis of clearance of HB-EGF in adult and newborn rats PMID: 16364500
    14. 5-HT2A receptor induces ERK phosphorylation and proliferation through TACE enzyme activation and HB-EGF shedding in mesangial cells PMID: 16737974
    15. extracellular superoxide dismutase is related to the epidermal growth factor (EGF) signaling in two ways, competition for HSPG with heparin-binding-EGF and as an ROS scavenger PMID: 16753836
    16. It is suggested that the increased expression of HB-EGF and the inhibition of glial cell swelling may be parts of a protective role of HB-EGF in the ischemic retina. PMID: 16806064
    17. Juxtacrine activation of EGFR by membrane-anchored HB-EGF may play an important role in the regulation of tight junction proteins and transepithelial resistance. PMID: 17855771
    18. Collectively, these data demonstrate that membrane-anchored HB-EGF is targeted to the inner nuclear membrane via a retrograde membrane trafficking pathway. PMID: 18299347
    19. Pro-inflammatory stimuli induce the release of HB-EGF from mesenchyme cells, which stimulates DNA-replication in initiated/premalignant hepatocytes PMID: 18929421
    20. rapid HA accumulation after epidermal wounding occurs through a mechanism requiring cleavage of HB-EGF and activation of EGFR signaling. PMID: 19225541
    21. mechanical stretch promotes fetal type II cell differentiation via ectodomain shedding of HB-EGF and TGF-alpha PMID: 19237431
    22. Hbegf is a potent dilator of terminal mesenteric arterioles. PMID: 19389413

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  • 亞細(xì)胞定位：
    [Heparin-binding EGF-like growth factor]: Secreted, extracellular space. Note=Mature HB-EGF is released into the extracellular space and probably binds to a receptor.; [Proheparin-binding EGF-like growth factor]: Cell membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Most abundant in skeletal muscle, lung, spleen brain and heart.
  • 數(shù)據(jù)庫(kù)鏈接：