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  4. Rat intestinal fatty acid binding protein,iFABP ELISA Kit

Rat intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名稱：
    大鼠腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08026r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒（CSB-E08026r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的FABP2含量。FABP2 即脂肪酸結(jié)合蛋白 2，主要在小腸表達(dá)。它可結(jié)合脂肪酸并參與細(xì)胞內(nèi)脂質(zhì)代謝。研究機(jī)制方面，其結(jié)構(gòu)使其能特異性結(jié)合脂肪酸，轉(zhuǎn)運(yùn)至代謝位點(diǎn)，與肥胖、糖尿病等代謝疾病發(fā)病機(jī)制密切相關(guān)，是潛在治療靶點(diǎn)。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，被廣泛用于腸道屏障功能障礙、缺血再灌注損傷及炎癥性腸病等研究的生物標(biāo)志物；可靈活應(yīng)用于動物模型腸道損傷動態(tài)監(jiān)測、藥物干預(yù)效果評估或疾病機(jī)制研究；適用于實(shí)驗(yàn)室常規(guī) ELISA 檢測系統(tǒng)，為消化道相關(guān)科研提供穩(wěn)定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.312 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %89-101
    1:2Average %93
    Range %87-99
    1:4Average %90
    Range %85-95
    1:8Average %104
    Range %98-108
  • 回收率：
    The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)9389-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.695 2.671 2.683 2.536
    401.915 1.901 1.908 1.761
    201.306 1.334 1.320 1.173
    100.884 0.899 0.892 0.745
    50.517 0.537 0.527 0.380
    2.50.375 0.384 0.380 0.233
    1.250.211 0.233 0.222 0.075
    00.145 0.149 0.147 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 基因功能參考文獻(xiàn)：
    1. Study characterized internal-water exchange and associated conformational dynamics in rIFABP by recording the water 2H and 17O MRD profiles for the apo and palmitate-bound forms PMID: 26012957
    2. This work highlights the importance of the beta-barrel of IFABP for its specific functions. PMID: 25311169
    3. the portal region of IFABP penetrates deeply into the phospholipid bilayer, especially when CL-containing vesicles are employed. The orientation of the protein and the degree of penetration were highly dependent on the lipid composition PMID: 24148314
    4. n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARgamma pathway, and then upregulating tight junction protein expression. PMID: 22554584
    5. Conjugated linoleic acid can improve insulin resistance of obese rat and increase the expression of fatty acid binding protein 2 mRNA. PMID: 16921753
    6. Delta 78 Delta beta-barrel motif retains substantial beta-sheet content and preserves tertiary interactions, displaying cooperative unfolding and binding activity. Most strikingly, this construct adopts a remarkably stable dimeric structure in solution. PMID: 19844951
    7. simulations of apo and holo-fatty acid binding protein: structure and dynamics of protein, ligand and internal water PMID: 11812142
    8. Exchanging the alpha-helical domain of intestinal FABP (IFABP) for that of liver FABP drastically modifies the collisional mechanism characteristic of IFABP. PMID: 15035630
    9. Increased concentrations of H-FABP significantly correlated with the concentrations of TNF- alpha in chronic heart failuare. PMID: 15277733
    10. An examination of types of intermediates that are observed during the folding of this protein. PMID: 15723553
    11. ionic interactions between IFABP and membranes appear to play a primary role in the process of fatty acid transfer to membranes, and hydrophobic interactions can also modulate the rates of ligand transfer PMID: 15863832
    12. FABP2 gene polymorphism is associated in patients diagnosed with carotid artery atheroscelrosis. PMID: 16013194
    13. analysis of intestinal fatty acid-binding protein stability and unfolding PMID: 16162507
    14. there may be different collisional complex structures for holo- and apo-IFABP PMID: 16551626
    15. The Gly121Val mutation of IFABP slows the formation of a normal nucleating site, not only slowing overall folding, but also allowing intermediates in regions distant from the mutation to be experimentally observed. PMID: 17615232
    16. alphaI-helix is crucial for IFABP collisional FA transfer, and further indicate the participation of the alphaII-helix in the formation of a protein-membrane "collisional complex". PMID: 18284926
    17. A structural explanation of the observed affinity of rat I-FABP for different drugs based on spectroscopic data and modeling experiments is presented.immunocytochemistry has been used to probe the expression of I-FABP in a cell culture model. PMID: 19160019
    18. Delta98Delta (encompassing residues 29-126) emerges as a monomeric variant of intestinal fatty acid binding protein that folds properly, retaining functional activity, despite lacking extensive stretches involved in the closure of the beta-barrel. PMID: 19309727

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 組織特異性：
    Expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:25598

    UniGene: Rn.91358