Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶聯(lián)免疫試劑盒（CSB-E08320r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Lp-PLA2含量。Lp-PLA2（脂蛋白相關(guān)磷脂酶A2）是一種與動(dòng)脈粥樣硬化和心血管疾病密切相關(guān)的標(biāo)志物，由血管內(nèi)膜中的巨噬細(xì)胞、T細(xì)胞和肥大細(xì)胞分泌。其水平高低與腦卒中和心血管疾病風(fēng)險(xiǎn)密切相關(guān)，通過水解氧化低密度脂蛋白中的氧化磷脂，生成脂類促炎物質(zhì)，促進(jìn)動(dòng)脈粥樣硬化。試劑盒檢測范圍為0.78 IU/mL-50 IU/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)大鼠模型血清或血漿樣本的Lp-PLA2水平監(jiān)測，例如心血管疾病動(dòng)物模型的病理機(jī)制研究、藥物干預(yù)效果評(píng)估或炎癥相關(guān)代謝調(diào)控實(shí)驗(yàn)，為體外實(shí)驗(yàn)提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Lp-PLA2
Uniprot No.：

Q5M7T7
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.78 IU/mL-50 IU/mL
靈敏度：

0.195 IU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	105
	Range %	100-110
1:4	Average %	86
	Range %	82-90
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.