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  4. Rat macrophage inflammatory protein 1β,MIP-1β ELISA kit

Rat macrophage inflammatory protein 1β,MIP-1β ELISA kit

  • 中文名稱：
    大鼠巨噬細(xì)胞炎癥蛋白1β(MIP-1β/CCL4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07421r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠巨噬細(xì)胞炎癥蛋白1β(MIP-1β/CCL4)酶聯(lián)免疫試劑盒（CSB-E07421r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL4含量。CCL4即趨化因子配體4，在免疫調(diào)節(jié)等生理過程中起重要作用。其背景與免疫細(xì)胞的招募和活化密切相關(guān)。研究機制上，CCL4通過與相應(yīng)受體結(jié)合，引導(dǎo)免疫細(xì)胞遷移、調(diào)控免疫反應(yīng)，在炎癥、腫瘤等疾病病理進程中有相關(guān)研究。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于炎癥相關(guān)動物模型構(gòu)建、免疫調(diào)控機制解析、藥理學(xué)研究中生物標(biāo)志物動態(tài)監(jiān)測等科研場景，為研究者探索巨噬細(xì)胞活化、趨化因子網(wǎng)絡(luò)交互作用等基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4C-C motif chemokine 4 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Small-inducible cytokine A4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %106
    Range %101-112
    1:2Average %94
    Range %90-98
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %92-104
  • 回收率：
    The recovery of rat MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9387-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.564 2.476 2.520 2.375
    1002.174 2.014 2.094 1.949
    501.418 1.372 1.395 1.250
    250.917 0.973 0.945 0.800
    12.50.555 0.589 0.572 0.427
    6.250.394 0.417 0.406 0.261
    3.120.289 0.273 0.281 0.136
    00.148 0.141 0.145
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Monokine with inflammatory and chemokinetic properties.
  • 基因功能參考文獻(xiàn)：
    1. Tong Luo Jiu Nao injection inhibits MIP-1beta expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation and inhibitors microglial cell proliferation. PMID: 22366434
    2. Early and persistent up-regulation of CCL4/CCR5 signaling during epileptogenesis suggests that CCL4 signaling, rather than CCL2 signaling, could have a role in the epileptogenic process. PMID: 22353418
    3. this study we present data on long term increase in CCL4 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
    4. During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of MIP-1 alpha. PMID: 12196270
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫鏈接：