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  4. Rat matrix metallopeptidase 8 (neutrophil collagenase) (MMP8) ELISA kit

Rat matrix metallopeptidase 8 (neutrophil collagenase) (MMP8) ELISA kit

  • 中文名稱：
    大鼠基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8/Collagenase II)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07406r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8/Collagenase II)酶聯(lián)免疫試劑盒（CSB-E07406r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP8含量。MMP8 即基質(zhì)金屬蛋白酶 8，在生理上參與組織重塑等過(guò)程。其異常表達(dá)與多種疾病相關(guān)，如炎癥、腫瘤等。研究機(jī)制主要圍繞其在疾病發(fā)生發(fā)展中對(duì)細(xì)胞外基質(zhì)的降解作用，以及與其他信號(hào)通路的交互，為相關(guān)疾病治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，可滿足體外實(shí)驗(yàn)或動(dòng)物模型中MMP - 8表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)需求，例如關(guān)節(jié)炎模型、肺部炎癥研究或創(chuàng)傷修復(fù)機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Mmp8Neutrophil collagenase ELISA Kit; EC 3.4.24.34 ELISA Kit; Matrix metalloproteinase-8 ELISA Kit; MMP-8 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %90
    Range %85-94
    1:4Average %88
    Range %84-92
    1:8Average %102
    Range %98-108
  • 回收率：
    The recovery of rat MMP8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8480-88
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.620 2.459 2.540 2.363
    102.056 2.146 2.101 1.924
    51.383 1.432 1.408 1.231
    2.50.825 0.801 0.813 0.636
    1.250.509 0.518 0.514 0.337
    0.6250.417 0.405 0.411 0.234
    0.3120.251 0.257 0.254 0.077
    00.180 0.173 0.177
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Can degrade fibrillar type I, II, and III collagens.
  • 基因功能參考文獻(xiàn)：
    1. Simvastatin significantly blocked cigarette smoke induced up-regulation of MMP-8 and -9, but had no effect on MMP-12, TIMP-1 and TIMP-4 expression in rat COPD model. PMID: 28260878
    2. Study showed that E. faecalis strains ATCC 29212 or ATCC 700802 could induce local IL-1beta and MMP-8 expression in persistent apical periodontitis; compared with noncytolytic E. faecalis, cytolytic E. faecalis may cause more severe local inflammation and tissue destruction PMID: 26055433
    3. hydrogen sulfide downregulated protein and mRNA expression of MMP-8, MMP-13, and TIMP-1 in rats with volume overload; this effect might be involved in the amelioration of ventricular structural remodeling and cardiac function PMID: 23974514
    4. MMP-8, MMP-9 and MMP-12 gene and protein expression increased profoundly (p < 0.01), and MMP-13 mRNA and MMP-2 mRNA and protein modestly (p < 0.001) in the anastomoses. PMID: 23619615
    5. Elevated levels of MMP-8 in CSF were found following spinal cord injury. PMID: 22687332
    6. Mmp8/14 expression seem important in the tissue destruction during periodontal disease in type 1 diabetes. PMID: 21826658
    7. These results demonstrate that superphysiologic levels of the proteinase MMP-8 can result in decreased collagen and lead to impaired wound healing. PMID: 21875735
    8. MMP-8 is involved in sensory nerve growth within the interstitial CoL matrix through modulation by the axonal guidance molecules and/or extracellular matrix components PMID: 21697883
    9. related to the development of periradicular lesions PMID: 21447140
    10. Venous pressure elevation (induced by acute venular occlusion) increases MMP-1, MMP-9, and MMP-8 enzymatic activity which may contribute to inflammation and endothelial dysfunction associated with this disease by influencing critical surface receptors. PMID: 20923679
    11. MMP-2 and MMP-8 cooperate to degrade the cervical fibers, leading to cervical softening and expansion. PMID: 19923067
    12. Ibuprofen upregulates the expressions of collagenases including MMP-1, -8, -9, and -13. PMID: 19847888
    13. These results indicate that bradykinin at reperfusion triggers its cardioprotective signalling pathway through MMP-8-dependent transactivation of the EGFR. PMID: 19583703
    14. Results suggest that matrix metalloproteinase (MMP)-13, but not MMP-8 or TIMP-1, plays an important role in estrogen deficiency-induced bone loss. PMID: 15609084
    15. secretion of MMP-8 by neutrophils may play a critical role in resolving the fibrotic scar generated during cholestasis PMID: 16153442
    16. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMP8 during healing of a defect in bone. PMID: 18615687
    17. Expression of metalloproteinase-8 and -13 on articular cartilage is increased in a rat immobilized knee model. PMID: 19346731

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  • 亞細(xì)胞定位：
    Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Note=Stored in intracellular granules.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫(kù)鏈接：