Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit
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中文名稱:大鼠基質(zhì)金屬蛋白酶1(MMP-1/Collagenase I)酶聯(lián)免疫試劑盒
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貨號:CSB-E07416r
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠基質(zhì)金屬蛋白酶1(MMP-1/Collagenase I)酶聯(lián)免疫試劑盒(CSB-E07416r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP1含量。MMP1,即基質(zhì)金屬蛋白酶1,是一種鋅依賴性內(nèi)肽酶,參與細(xì)胞外基質(zhì)(ECM)的降解,與細(xì)胞遷移、炎癥和腫瘤侵襲等生理和病理過程密切相關(guān)。MMP1通過降解ECM中的膠原和蛋白聚糖,改變細(xì)胞微環(huán)境,促進(jìn)腫瘤的侵襲和轉(zhuǎn)移。研究MMP1的機(jī)制有助于開發(fā)針對腫瘤侵襲和轉(zhuǎn)移的治療策略。試劑盒檢測范圍為0.235 ng/ml- 15 ng/ml,靈敏度為0.058 ng/ml。適用于炎癥模型、組織工程研究、纖維化疾病機(jī)制探索等科研場景,能夠?yàn)樾难芗膊?、關(guān)節(jié)炎等動物模型的分子機(jī)制研究提供可靠數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.235 ng/ml - 15 ng/ml
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靈敏度:0.058 ng/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase?I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
89
Range %
85-94
1:2
Average %
92
Range %
87-98
1:4
Average %
95
Range %
90-102
1:8
Average %
100
Range %
96-109
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回收率:
The recovery of rat MMP-1/Collagenase?I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
84
80-89
EDTA plasma (n=4)
97
90-104
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
15
2.257
2.231
2.244
2.147
7.5
1.876
1.832
1.854
1.757
3.75
1.381
1.373
1.377
1.280
1.88
0.835
0.797
0.816
0.719
0.94
0.397
0.376
0.387
0.290
0.47
0.273
0.257
0.265
0.168
0.235
0.197
0.207
0.202
0.105
0
0.099
0.094
0.097
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Photoaging induced by long-term exposure to UV irradiation and amelioration by Skipjack tuna skin hydrolysates: Targeting inhibition of MAPK and NF-κB signaling hyperactivation D Xu,Journal of Functional Foods,2022
- Dietary Supplementation with Palmitoyl-Glucosamine Co-Micronized with Curcumin Relieves Osteoarthritis Pain and Benefits Joint Mobility E Gugliandolo,Animals,2020
- Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats G Shamlan,Arabian Journal for Science and Engineering,2020
- Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain Cordaro M, et al,Arthritis Research & Therapy,2019
- A novel composite formulation of palmitoylethanolamide and quercetin decreases inflammation and relieves pain in inflammatory and osteoarthritic pain models Britti D.et al,BMC Vet Res.,2017
- Adelmidrol, in combination with hyaluronic acid, displays increased anti-inflammatory and analgesic effects against monosodium iodoacetate-induced osteoarthritis in rats Rosanna Di Paola.et al,Arthritis Research & Therapy,2016
- Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:MMP1,全名為基質(zhì)金屬蛋白酶-1,是一種廣泛存在于人體組織中的酶類分子。MMP1主要參與膠原蛋白的降解和清除過程,在組織再生、炎癥、腫瘤轉(zhuǎn)移等多種生理和病理過程中發(fā)揮重要作用。最近的研究表明,MMP1在多種癌癥的發(fā)生和發(fā)展中發(fā)揮關(guān)鍵作用,成為了一個重要的治療靶點(diǎn)。此外,MMP1還與關(guān)節(jié)炎、動脈粥樣硬化等疾病的發(fā)生和發(fā)展密切相關(guān),成為了重要的生物標(biāo)志物和疾病預(yù)測因子。針對這些酶的抑制劑已成為研究的熱點(diǎn),可以用于疾病的預(yù)防和治療。
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功能:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
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亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix.
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蛋白家族:Peptidase M10A family
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數(shù)據(jù)庫鏈接:
UniGene: Rn.79007
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