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  4. Rat matrix metalloproteinase 10,MMP-10 ELISA kit

Rat matrix metalloproteinase 10,MMP-10 ELISA kit

  • 中文名稱：
    大鼠基質(zhì)金屬蛋白酶10(MMP-10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07414r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠基質(zhì)金屬蛋白酶10(MMP-10)酶聯(lián)免疫試劑盒（CSB-E07414r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP10含量。MMP10 即基質(zhì)金屬蛋白酶 10，在多種生物學(xué)過(guò)程如組織重塑、細(xì)胞遷移等中發(fā)揮作用。其異常表達(dá)與腫瘤生長(zhǎng)、侵襲和轉(zhuǎn)移等密切相關(guān)。研究機(jī)制主要聚焦于調(diào)控細(xì)胞外基質(zhì)降解，以及與其他信號(hào)通路相互作用等方面，是頗具潛力的治療靶點(diǎn)。試劑盒檢測(cè)范圍為80 pg/mL-2000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于炎癥性疾病機(jī)制探究、腫瘤轉(zhuǎn)移模型分析，以及心血管疾病或肝纖維化等科研領(lǐng)域的基礎(chǔ)研究，為探索MMP - 10的生物學(xué)功能提供可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
  • 縮寫：
    MMP10
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    80 pg/mL-2000 pg/mL
  • 靈敏度：
    50 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 85-99
    1:2 Average % 92
    Range % 90-103
    1:4 Average % 95
    Range % 87-105
    1:8 Average % 95
    Range % 87-99
  • 回收率：
    The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 88-100
    EDTA plasma (n=4) 95 89-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    2000 0.172 0.175 0.174
    800 0.376 0.379 0.378
    480 0.554 0.562 0.558
    200 0.882 0.897 0.890
    80 1.493 1.475 1.484
    0 2.404 2.452 2.428
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • 基因功能參考文獻(xiàn)：
    1. MMP-10 modulates corneal epithelial wound healing. PMID: 24339723
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫(kù)鏈接：