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  4. Rat matrix metalloproteinase 3,MMP-3 ELISA kit

Rat matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名稱：
    大鼠基質金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07410r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠基質金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒（CSB-E07410r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP3含量。MMP3，即基質金屬蛋白酶3，是一種在多種生理和病理過程中發(fā)揮關鍵作用的酶類分子。它在炎癥性疾病、腫瘤、動脈粥樣硬化和神經(jīng)系統(tǒng)疾病中扮演重要角色，能夠降解多種基質成分，并激活其他MMPs，參與組織重塑和疾病進程。研究顯示，MMP3的基因多態(tài)性與某些疾病的遺傳易感性相關，如食管鱗癌。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，本產品可廣泛應用于基礎醫(yī)學研究領域，如關節(jié)炎動物模型中的基質代謝研究、藥物干預下MMP - 3表達水平動態(tài)監(jiān)測，或腫瘤微環(huán)境中蛋白酶活性評估等實驗場景，為組織重塑機制探索及病理模型構建提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %90
    Range %87-93
    1:200Average %94
    Range %90-98
    1:400Average %95
    Range %91-99
    1:800Average %92
    Range %88-96
  • 回收率：
    The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9490-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.569 2.547 2.558 2.429
    502.236 2.213 2.225 2.096
    251.868 1.829 1.849 1.720
    12.51.367 1.387 1.377 1.248
    6.250.840 0.889 0.865 0.736
    3.130.377 0.387 0.382 0.253
    1.560.247 0.259 0.253 0.124
    00.128 0.130 0.129 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接：

    UniGene: Rn.32086