Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit
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中文名稱(chēng):大鼠髓過(guò)氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體(MPO-ANCA)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08675r
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠髓過(guò)氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體(MPO-ANCA)酶聯(lián)免疫試劑盒(CSB-E08675r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的MPO-ANCA含量。MPO-ANCA(髓過(guò)氧化物酶特異性抗中性粒細(xì)胞胞漿抗體)是一種與血管炎和腎小球腎炎密切相關(guān)的自身抗體。其研究機(jī)制主要涉及MPO分子被自身免疫性T/B細(xì)胞識(shí)別的抗原表位,以及易感HLA分子、補(bǔ)體活化、凝血和血小板等參與的免疫炎癥反應(yīng)。MPO-ANCA的致病性在動(dòng)物模型和臨床研究中得到證實(shí),其與疾病活動(dòng)度密切相關(guān),是治療的關(guān)鍵靶點(diǎn)。試劑盒檢測(cè)范圍為0.625 U/mL-40 U/mL,該產(chǎn)品適用于科研場(chǎng)景中血管炎動(dòng)物模型的抗體水平動(dòng)態(tài)監(jiān)測(cè)、疾病機(jī)制探索以及藥物干預(yù)效果評(píng)價(jià)等研究,為深入解析MPO - ANCA在免疫病理過(guò)程中的作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:N/A
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縮寫(xiě):MPO-ANCA
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, plasma
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檢測(cè)范圍:0.625 U/mL-40 U/mL
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靈敏度:0.156 U/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線(xiàn)性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-95 1:2 Average % 97 Range % 91-105 1:4 Average % 96 Range % 92-100 1:8 Average % 93 Range % 85-98 -
回收率:
The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-97 EDTA plasma (n=4) 91 84-96 -
標(biāo)準(zhǔn)曲線(xiàn):
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. U/ml OD1 OD2 Average Corrected 40 2.612 2.752 2.682 2.518 20 2.013 2.076 2.045 1.881 10 1.367 1.304 1.336 1.172 5 0.802 0.837 0.820 0.656 2.5 0.444 0.424 0.434 0.270 1.25 0.351 0.359 0.355 0.191 0.625 0.241 0.225 0.233 0.069 0 0.160 0.167 0.164 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The immunomodulatory effect of ivermectin on rat vasculitis model TS Sadeq, NH Mohammed, IS Arif,Immunopathologia Persa,2025
- Expression and significance of Fractalkine/CX3CL1 in MPO-AAV-associated glomerulonephritis rats J Ma,BMC nephrology,2024
- Persistent immune stimulation exacerbates genetically-driven myeloproliferative disorders via stromal remodeling Tripodo C.et al,Cancer Res,2017
相關(guān)問(wèn)答
Can you please confirm the following
1) Standard - Is it a recombinant or not? If it's recombinant, please let us know the origin. ( for ex;E-coli, bculovirus).
2) Antibodies → Please let us know whether the antibodies include NaN3, Thimerosal or 2-Me as preservative. If it have any preservatives, please give us the content(%)
3) Stop Solution → Please let us know the constituent and concentration. We would like to confirm about the toxicity.
CSB-E08675r
This kit is for quantitative determination of antibody.
Standard is crude extract from natural samples.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain NaN3, Thimerosal or 2-Me.
Pls let me know if you have any further questions. Thank you.
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