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  4. Rat neuropeptide Y (NPY) ELISA kit

Rat neuropeptide Y (NPY) ELISA kit

  • 中文名稱：
    大鼠神經(jīng)肽Y(NPY)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13431r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠神經(jīng)肽Y(NPY)酶聯(lián)免疫試劑盒（CSB-E13431r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NPY含量。NPY即神經(jīng)肽Y，是一種廣泛分布于中樞和外周神經(jīng)系統(tǒng)的神經(jīng)肽。它參與多種生理功能調(diào)節(jié)，如攝食、能量代謝、心血管活動等。研究機制方面，主要通過與不同亞型受體結(jié)合，激活下游信號通路來發(fā)揮作用，在相關(guān)疾病治療研究中是潛在靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于神經(jīng)生物學(xué)研究、代謝調(diào)節(jié)機制探索、藥物干預(yù)效果評價等科研領(lǐng)域。支持大鼠模型中的NPY動態(tài)監(jiān)測，例如神經(jīng)退行性疾病模型、肥胖癥研究或應(yīng)激反應(yīng)實驗中的生物標志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %92-104
    1:2Average %97
    Range %92-103
    1:4Average %93
    Range %89-95
    1:8Average %86
    Range %82-92
  • 回收率：
    The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-99
    EDTA plasma (n=4)105100-110
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.487 2.385 2.436 2.290
    501.629 1.527 1.578 1.432
    250.916 0.937 0.927 0.781
    12.50.574 0.565 0.570 0.424
    6.250.375 0.386 0.381 0.235
    3.120.261 0.250 0.256 0.110
    1.560.186 0.182 0.184 0.038
    00.146 0.145 0.146 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
  • 亞細胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
  • 蛋白家族：
    NPY family
  • 組織特異性：
    One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
  • 數(shù)據(jù)庫鏈接：