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  4. Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

  • 中文名稱：
    大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08788r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯(lián)免疫試劑盒（CSB-E08788r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的NFκB p65含量。NFκB p65是重要的轉(zhuǎn)錄因子，在細(xì)胞的炎癥、免疫、增殖和凋亡等多種生理病理過程中發(fā)揮關(guān)鍵作用。其激活機(jī)制多樣，與多條信號(hào)通路相互關(guān)聯(lián)。對(duì)其研究有助于深入了解疾病發(fā)病機(jī)制，為開發(fā)針對(duì)性療法如抗炎、抗腫瘤藥物等提供理論基礎(chǔ)。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，適用于基礎(chǔ)科研中炎癥機(jī)制研究、免疫調(diào)控實(shí)驗(yàn)或藥物干預(yù)效果評(píng)估，例如在動(dòng)物模型中分析NF-κB信號(hào)通路激活狀態(tài)，或探究氧化應(yīng)激、感染性疾病等實(shí)驗(yàn)條件下p65亞基的動(dòng)態(tài)表達(dá)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    NF-Kappa B,nuclear factor-kappa B
  • 縮寫：
    NFκB p65
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %86-92
    1:200Average %99
    Range %97-103
    1:400Average %89
    Range %85-93
    1:800Average %95
    Range %91-99
  • 回收率：
    The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.039 2.022 2.031 1.887
    501.334 1.326 1.330 1.186
    250.908 0.902 0.905 0.761
    12.50.566 0.557 0.562 0.418
    6.250.358 0.364 0.361 0.217
    3.120.291 0.281 0.286 0.142
    1.560.243 0.232 0.238 0.094
    00.145 0.143 0.144 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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