Rat omentin ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠網(wǎng)膜素(omentin)酶聯(lián)免疫試劑盒（CSB-E09747r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的omentin含量。omentin是一個研究靶點。其背景上，它可能在人體生理過程中具有一定作用。研究機(jī)制方面，目前具體情況暫不明確，但推測它或參與脂肪代謝、炎癥調(diào)節(jié)等生理活動，后續(xù)還需更多研究探索其在各生理及病理機(jī)制中的具體角色。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可利用開展脂肪代謝機(jī)制研究、能量代謝相關(guān)疾病模型評估、藥物干預(yù)效果分析等實驗，為探索網(wǎng)膜素在能量穩(wěn)態(tài)調(diào)控中的功能提供定量檢測支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

omentin
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

回收率：

The recovery of rat omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.